来自极端嗜盐古菌日本嗜盐嗜盐菌TR-1株的编码2Fe-2S铁氧化还原蛋白基因的分子克隆。

Molecular cloning of the gene encoding a 2Fe-2S ferredoxin from extremely halophilic archaeon Haloarcula japonica strain TR-1.

作者信息

Ikeda A, Ichimata T, Sugimori D, Nakamura S

机构信息

Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Nucleic Acids Symp Ser. 1997(37):109-10.

PMID:9586023

Abstract

A ferredoxin was purified from extremely halophilic archaeon Haloarcula japonica strain TR-1, and then characterized. The Ha. japonica ferredoxin proved to contain a 2Fe-2S cluster. A part of the gene encoding the ferredoxin was amplified by PCR. Subsequently, the entire ferredoxin gene was cloned from chromosomal DNA of Ha. japonica using the PCR product as a probe. The structural gene consisted of an open reading frame of 387 nucleotides. The deduced amino acid sequence showed 89-98% identities with those of the ferredoxins from other extremely halophilic archaea.

摘要

从极端嗜盐古菌日本嗜盐碱杆菌TR-1菌株中纯化出一种铁氧化还原蛋白，然后对其进行表征。结果表明，日本嗜盐碱杆菌铁氧化还原蛋白含有一个2Fe-2S簇。通过PCR扩增了编码该铁氧化还原蛋白的部分基因。随后，以PCR产物为探针，从日本嗜盐碱杆菌的染色体DNA中克隆出完整的铁氧化还原蛋白基因。该结构基因由一个387个核苷酸的开放阅读框组成。推导的氨基酸序列与其他极端嗜盐古菌的铁氧化还原蛋白的氨基酸序列具有89%-98%的同一性。