Saito Yoshio, Kanatani Keiichiro, Ochi Yuji, Okamoto Akimitsu, Saito Isao
NEWCAT Institute, Faculty of Engineering, Nihon University and SORST, Japan Science and Technology Corporation, Tamura, Koriyama 963-8642, Japan.
Nucleic Acids Symp Ser (Oxf). 2004(48):243-4. doi: 10.1093/nass/48.1.243.
We have devised novel pyrene-labeled BDF nucleosides, (Py)U, (Py)C, (Py)deazaA, and (Py)A. BDF probes containing these fluorescent nucleosides selectively emit fluorescence only when the base opposite the BDF base is a target base. Oligonucleotides containing these BDF nucleosides act as effective reporter probes for homogeneous SNP typing of DNA samples.
我们设计了新型的芘标记的BDF核苷,即(Py)U、(Py)C、(Py)脱氮腺苷和(Py)A。含有这些荧光核苷的BDF探针仅在与BDF碱基相对的碱基为目标碱基时选择性地发出荧光。含有这些BDF核苷的寡核苷酸可作为DNA样品均相SNP分型的有效报告探针。