Dual-labeled oligonucleotide probe for sensing adenosine via FRET: a novel alternative to SNPs genotyping.

作者信息

Saito Yoshio, Bag Subhendu Sekhar, Kusakabe Yuichi, Nagai Chiharu, Matsumoto Katsuhiko, Mizuno Erika, Kodate Satoshi, Suzuka Isamu, Saito Isao

机构信息

Department of Materials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.

出版信息

Chem Commun (Camb). 2007 Jun 7(21):2133-5. doi: 10.1039/b618465k. Epub 2007 Feb 28.

DOI:10.1039/b618465k

PMID:17520113

Abstract

A novel FRET based strategy for DNA sequence analysis utilising base-discriminating fluorescence (BDF) nucleoside, (Py)U/(2-Ant)U, as donor in the dual-labelled oligonucleotide probe is reported; a selective/specific emission from acceptor, was observed upon excitation at the donor, only when the opposite base of the "smart" fluorescently labeled BDF nucleoside, (Py)U/(2-Ant)U, is adenine on the complementary target sequence.

摘要