含恶嗪-芘发色团的单标记和双标记碱基区分荧光寡核苷酸探针。

Singly and doubly labeled base-discriminating fluorescent oligonucleotide probes containing oxo-pyrene chromophore.

机构信息

Bioorganic Chemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Guwahati 781039, Assam, India.

出版信息

Bioorg Med Chem Lett. 2010 Jun 1;20(11):3227-30. doi: 10.1016/j.bmcl.2010.04.063. Epub 2010 Apr 19.

DOI:10.1016/j.bmcl.2010.04.063

PMID:20452214

Abstract

We have developed new oxo-pyrene labeled fluorescent nucleoside, (Oxo-Py)U which showed a strong fluorescence dependency on solvent polarity at long wavelength. The designed singly and doubly (Oxo-Py)U labeled fluorescent oligonucleotide probes were found highly efficient for the discrimination of A and consecutive AA bases of target DNA opposite to the labeled base via generation of enhanced fluorescence signal.

摘要

我们开发了新型的氧杂芘标记荧光核苷，（Oxo-Py）U，其在长波长处表现出对溶剂极性的强烈荧光依赖性。设计的单和双（Oxo-Py）U 标记荧光寡核苷酸探针被发现通过增强荧光信号的产生，对标记碱基相反的靶 DNA 中的 A 和连续 AA 碱基具有高效的区分能力。