Mukumoto Kosuke, Nojima Takahiko, Takenaka Shigeori
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Nucleic Acids Symp Ser (Oxf). 2004(48):251-2. doi: 10.1093/nass/48.1.251.
Ferrocenyl carbodiimide (FcCDI) was newly synthesized as a ferrocenylation reagent for single stranded DNA. FcCDI could attach to oligodeoxyribonucleotides (ODN) through a covalent bond with thymine and guanine bases. The reactivity of thymine with FcCDI was higher than that of guanine, but independent of their location on the DNA sequence. After a single stranded DNA was treated with FcCDI, resulting ODN could be detected electrochemically by a redox signal deriving from the ferrocenyl moiety with a DNA probe-immobilized electrode. These results suggest that the labeling of DNA with FcCDI can be applied to rapid analysis of electrochemical genosensors.
二茂铁基碳二亚胺（FcCDI）作为一种用于单链DNA的二茂铁化试剂被新合成出来。FcCDI能够通过与胸腺嘧啶和鸟嘌呤碱基形成共价键而连接到寡脱氧核糖核苷酸（ODN）上。胸腺嘧啶与FcCDI的反应活性高于鸟嘌呤，但与它们在DNA序列上的位置无关。用FcCDI处理单链DNA后，所得的ODN可以通过固定有DNA探针的电极上源自二茂铁基部分的氧化还原信号进行电化学检测。这些结果表明，用FcCDI对DNA进行标记可应用于电化学基因传感器的快速分析。
