Mukumoto Kosuke, Nojima Takahiko, Sato Shinobu, Waki Michinori, Takenaka Shigeori
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Anal Sci. 2007 Jan;23(1):115-9. doi: 10.2116/analsci.23.115.
Ferrocenyl carbodiimide (1) could be used for the direct labeling of synthetic RNA and expressed mRNA in vitro with the electrochemically active ferrocene moieties. These RNAs modified by 1 could be detected electrochemically coupled with a DNA probe-immobilized electrode. After hybridization of 1.1 Kb mRNA modified by 1 with the DNA probe-immobilized electrode, the peak charge observed by an Osteryoung square wave voltammetry (SWV) measurement correlated well with the concentration of mRNA, having a detection limit at the sub nanogram level.
