Mukumoto Kosuke, Nojima Takahiko, Takenaka Shigeori
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):231-2. doi: 10.1093/nass/49.1.231.
Ferrocenyl carbodiimide (FCDI) was allowed to react with thymine or guanine bases of single stranded DNA in borate buffer (pH 9.5) at 37 degrees C for a short period of time. The reactivity of FCDI with the guanine bases was smaller than that with the thymine ones and the prolongation of the reaction time resulted in a lower yield. The reactivity for double stranded oligonucleotide was extremely decreased from that for the single stranded one. One base mismatched double stranded oligonucleotides could react with FCDI, albeit slowly. These results suggested that the reactivity of FCDI was variable depending on the kind of bases and the oligonucleotide structure.
在37摄氏度下，使二茂铁基碳二亚胺（FCDI）在硼酸盐缓冲液（pH 9.5）中与单链DNA的胸腺嘧啶或鸟嘌呤碱基短时间反应。FCDI与鸟嘌呤碱基的反应活性低于与胸腺嘧啶碱基的反应活性，且反应时间延长会导致产率降低。双链寡核苷酸的反应活性与单链寡核苷酸相比大幅降低。一个碱基错配的双链寡核苷酸也能与FCDI反应，尽管反应缓慢。这些结果表明，FCDI的反应活性因碱基种类和寡核苷酸结构而异。
