金属介导碱基对的结构和物理化学特征。

Structural and physicochemical features on the metal-mediated base pairs.

作者信息

Tanaka Yoshiyuki, Oda Shuji, Yamaguchi Hiroshi, Haruta Kaichiro, Kawamura Takuya, Kondo Yoshinori, Uchiyama Tomomi, Takashi Miura, Takeuchi Hideo, Torigoe Hidetaka, Kojima Chojiro, Ono Akira

机构信息

Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2007(51):75-6. doi: 10.1093/nass/nrm038.

DOI:10.1093/nass/nrm038

PMID:18029593

Abstract

The chemical structure of the mercury-mediated T-T pair (T-Hg(I)I-T) was determined with (15)N NMR spectroscopy. In order to determine the chemical structure of the T-Hg(I)I-T pair, (15)N-(15)N J-coupling across a metal center (2JNN) was employed. Notably, this is the first observation of (2)J(NN) in a biological macromolecule (DNA duplex). This pairing mode was found to be a irregular metal ion-binding mode for DNA and RNA molecules, in which imino proton-metal exchange processes are included. Accordingly, (2)J(NN) is highly important for the determination of the chemical structures of metal-mediated base pairs.

摘要

利用（15）N核磁共振光谱法确定了汞介导的T-T对（T-Hg（I）I-T）的化学结构。为了确定T-Hg（I）I-T对的化学结构，采用了跨金属中心的（15）N-（15）N J耦合（2JNN）。值得注意的是，这是在生物大分子（DNA双链体）中首次观察到（2）J（NN）。发现这种配对模式是DNA和RNA分子的一种不规则金属离子结合模式，其中包括亚氨基质子-金属交换过程。因此，（2）J（NN）对于确定金属介导的碱基对的化学结构非常重要。