Aiba Yuichiro, Mori Masao, Yamamoto Yoji, Komiyama Makoto
Research center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):277-8. doi: 10.1093/nass/49.1.277.
In order to hydrolyze double-stranded DNA efficiently at the target site, two pseudo-complementary peptide nucleic acids (pcPNAs) bearing phosphate group were combined with Ce(IV)/EDTA complex (EDTA = ethylenediamine-N,N,N',N'-tetraacetate). The phosphate groups as metal-binding ligands were placed near the target site, and concentrated the Ce(IV) complex thereto. As the result, the site-selective hydrolysis was notably promoted, compared with the scission by the cutters involving unmodified pcPNAs.
为了在靶位点高效水解双链DNA，将两个带有磷酸基团的假互补肽核酸（pcPNA）与Ce(IV)/EDTA复合物（EDTA = 乙二胺-N,N,N',N'-四乙酸）结合。作为金属结合配体的磷酸基团被置于靶位点附近，并将Ce(IV)复合物富集于此。结果表明，与涉及未修饰pcPNA的切割剂切割相比，位点选择性水解得到显著促进。
