Manipulation of double-stranded DNA by artificial restriction enzyme composed of Ce(IV)/EDTA and PNA.
作者信息
Yamamoto Yoji, Mori Masao, Tomita Takafumi, Zhou Jing-Min, Komiyama Makoto
机构信息
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
出版信息
Nucleic Acids Symp Ser (Oxf). 2004(48):279-80. doi: 10.1093/nass/48.1.279.
PMID:17150587
Abstract
Through the invasion of pseudo-complementary PNA (pePNA) to double-stranded DNA, gap-like structures were formed at predetermined sites in both strands of PBR322 plasmid DNA. These gap-like sites were selectively hydrolyzed by Ce(IV)/EDTA complex, and two designed fragments were obtained. Furthermore, the scission fragment by this artificial restriction enzyme was successfully ligated with foreign DNA.
摘要
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