Andrews P W, Meyer L J, Bednarz K L, Harris H
Hybridoma. 1984;3(1):33-9. doi: 10.1089/hyb.1984.3.33.
From a series of hybridomas that produced monoclonal antibodies reactive with the surface of human embryonal carcinoma cells, two that specifically recognized determinants of the liver/bone/kidney isozyme of alkaline phosphatase were isolated. They did not cross-react with the intestinal or placental isozymes. Phylogenetic studies revealed that both antibodies cross-reacted strongly with liver alkaline phosphatase from higher primates, but exhibited marked differences in their respective cross-reactions with liver alkaline phosphatase from other mammalian species.
从一系列产生与人类胚胎癌细胞表面发生反应的单克隆抗体的杂交瘤中，分离出两种能特异性识别碱性磷酸酶肝/骨/肾同工酶决定簇的抗体。它们与肠型或胎盘型同工酶不发生交叉反应。系统发育研究表明，这两种抗体都能与高等灵长类动物的肝脏碱性磷酸酶发生强烈交叉反应，但它们与其他哺乳动物物种的肝脏碱性磷酸酶的交叉反应存在显著差异。
