Accardo Antonella, Tesauro Diego, Mangiapia Gaetano, Pedone Carlo, Morelli Giancarlo
Department of Biological Sciences, CIRPeB University of Naples Federico II, & IBB CNR, Via Mezzocannone 16, 80134 Naples, Italy.
Biopolymers. 2007;88(2):115-21. doi: 10.1002/bip.20648.
The self-assembling behavior, at physiological pH, of the amphiphile peptide (C18)(2)L5CCK8 in nanostructures is reported. Stable aggregates presenting a critical micellar concentration of 2 x 10(-6) mol kg(-1), and characterized by water exposed CCK8 peptide in beta-sheet conformation, are obtained. Small angle neutron scattering experiments are indicative for a 3D structure with dimensions > or =100 nm. AFM images confirm the presence of nanostructures. Fluorescence experiments indicating the sequestration of pyrene, chosen as drug model, and the anticancer Doxorubicin within the nanostructures are reported.
报道了两亲性肽（C18）（2）L5CCK8在生理pH值下于纳米结构中的自组装行为。获得了临界胶束浓度为2×10^(-6) mol kg^(-1)的稳定聚集体，其特征是β-折叠构象的CCK8肽暴露于水相。小角中子散射实验表明存在尺寸≥100 nm的三维结构。原子力显微镜图像证实了纳米结构的存在。还报道了荧光实验，该实验表明作为药物模型的芘以及抗癌药物阿霉素在纳米结构中被隔离。
