Zhang C W
Department of Microbiology, Jiangxi Medical College, Nanchang.
Zhonghua Jie He He Hu Xi Za Zhi. 1991 Dec;14(6):325-7, 375.
The specific PPD IgG antibodies in serum of patients with pulmonary tuberculosis and its reactivity to PPD of Mycobacterium tuberculosis M. bovis and M. avium were detected by ELISA. The results showed that the antibody level and positive rate of patients with pulmonary tuberculosis significantly increased than those of normal subjects. Specific antibody level and its positive rate were higher in hospitalized patients than those treated in clinic and both were higher in sputum positive cases than in sputum negative one. There was cross-reactivity among three different PPD. Absorption black test indicated that M. tuberculosis PPD had good specificity. PPD should be further purified in order to make ELISA as a useful serodiagnostic tool.
采用酶联免疫吸附测定法（ELISA）检测肺结核患者血清中特异性结核菌素纯蛋白衍生物（PPD）IgG抗体及其对结核分枝杆菌、牛分枝杆菌和鸟分枝杆菌PPD的反应性。结果显示，肺结核患者的抗体水平和阳性率显著高于正常受试者。住院患者的特异性抗体水平及其阳性率高于门诊治疗患者，痰涂片阳性患者两者均高于痰涂片阴性患者。三种不同PPD之间存在交叉反应性。吸收阻断试验表明结核分枝杆菌PPD具有良好的特异性。为使ELISA成为一种有用的血清学诊断工具，PPD应进一步纯化。
