Inokuma Hisashi, Makino Takashi, Kabeya Hidenori, Nogami Sadao, Fujita Hiromi, Asano Makoto, Inoue Satoshi, Maruyama Soichi
Faculty of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan.
Vet Parasitol. 2007 Apr 10;145(1-2):186-9. doi: 10.1016/j.vetpar.2006.11.002. Epub 2006 Dec 8.
Numbers of feral raccoon; the possible reservoir animal of Ehrlichia and Anaplasma, are increasing in Japan. Thus serological methods were utilized to examine Ehrlichia and Anaplasma infection in raccoons from Kanagawa Prefecture, Japan. By using an indirect immunofluorescence assay, among 187 feral raccoons examined, 1 (0.5%) serologically reacted with Ehrlichia canis, 3 (1.6%) with Ehrlichia chaffeensis and 1 (0.5%) with Anaplasma phagocytophilum with the titers of 1:40 or more. Although screening PCR for Ehrlichia and Anaplasma species failed to detect the presence of ehrlichial DNA in serum samples, results of the serological tests suggested that the feral raccoons might be infected with some species of Ehrlichia and Anaplasma.
日本野生浣熊（埃立克体属和无形体属潜在的储存宿主动物）的数量正在增加。因此，本研究利用血清学方法检测了来自日本神奈川县的浣熊的埃立克体属和无形体属感染情况。通过间接免疫荧光试验，在检测的187只野生浣熊中，1只（0.5%）血清学上与犬埃立克体发生反应，3只（1.6%）与查菲埃立克体发生反应，1只（0.5%）与嗜吞噬细胞无形体发生反应，效价均为1:40或更高。尽管针对埃立克体属和无形体属物种的筛查PCR未能在血清样本中检测到埃立克体DNA的存在，但血清学检测结果表明，野生浣熊可能感染了某些埃立克体属和无形体属物种。
