Owen R L, Bhalla D K
Am J Anat. 1983 Oct;168(2):199-212. doi: 10.1002/aja.1001680207.
M cells in Peyer's patch follicle epithelium endocytose and transport luminal materials to intraepithelial lymphocytes. We examined (1) enzymatic characteristics of the epithelium covering mouse and rat Peyer's patches by using cytochemical techniques, (2) distribution of lectin-binding sites by peroxidase-labeled lectins, and (3) anionic site distribution by using cationized ferritin to develop a profile of M cell surface properties. Alkaline phosphatase activity resulted in deposits of dense reaction product over follicle surfaces but was markedly reduced over M cells, unlike esterase which formed equivalent or greater product over M cells. Concanavalin A, ricinus communis agglutinin, wheat germ agglutinin and peanut agglutinin reacted equally with M cells and with surrounding enterocytes over follicle surfaces. Cationized ferritin distributed in a random fashion along microvillus membranes of both M cells and enterocytes, indicating equivalent anionic site distribution. Staining for alkaline phosphatase activity provides a new approach for distinguishing M cells from enterocytes at the light microscopic level. Identical binding of lectins indicates that M cells and enterocytes share common glycoconjugates even though molecular groupings may differ. Lectin binding and anionic charge similarities of M cells and enterocytes may facilitate antigen sampling by M cells of particles and compounds that adhere to intestinal surfaces in non-Peyer's patch areas.
派尔集合淋巴结滤泡上皮中的M细胞通过胞吞作用将腔内物质转运至上皮内淋巴细胞。我们研究了：(1) 运用细胞化学技术检测覆盖小鼠和大鼠派尔集合淋巴结的上皮的酶学特性；(2) 用过氧化物酶标记的凝集素检测凝集素结合位点的分布；(3) 用阳离子铁蛋白检测阴离子位点分布，以建立M细胞表面特性的概况。碱性磷酸酶活性导致在滤泡表面有密集反应产物沉积，但在M细胞上明显减少，这与酯酶不同，酯酶在M细胞上形成的产物量相当或更多。伴刀豆球蛋白A、蓖麻凝集素、麦胚凝集素和花生凝集素与M细胞以及滤泡表面周围的肠上皮细胞反应程度相同。阳离子铁蛋白随机分布于M细胞和肠上皮细胞的微绒毛膜上，表明阴离子位点分布相同。碱性磷酸酶活性染色为在光学显微镜水平区分M细胞和肠上皮细胞提供了一种新方法。凝集素的相同结合表明，尽管分子分组可能不同，但M细胞和肠上皮细胞共享共同的糖缀合物。M细胞和肠上皮细胞在凝集素结合和阴离子电荷方面的相似性可能有助于M细胞对非派尔集合淋巴结区域肠道表面附着的颗粒和化合物进行抗原采样。
