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[在多碳水化合物培养基中对乳糖的假阳性反应]

[A false positive reaction to lactose in polycarbohydrate media].

作者信息

Podpletennaia I M

出版信息

Lab Delo. 1991(5):62-4.

PMID:1715952
Abstract

In combined media for primary identification Salmonella typhi are detectable by the lactose test only within a certain range of proteolytic activities, which fact is explained by specific features of these media. Reduced proteolytic activity and thiosulfate reductase activity in S. typhi cultures resulted in false-positive lactose test and false-positive hydrogen sulfide production test, this leading to identification of these cultures as Escherichia in accordance with the universally acknowledged classification scheme. Taking this feature into consideration, the author has additionally isolated 20 typical S. typhi strains of the 22 cultures isolated in the laboratory.

摘要

在用于初步鉴定的复合培养基中,仅在特定的蛋白水解活性范围内,才能通过乳糖试验检测到伤寒沙门氏菌,这一事实可由这些培养基的特定特性来解释。伤寒沙门氏菌培养物中蛋白水解活性和硫代硫酸盐还原酶活性的降低导致乳糖试验假阳性和硫化氢产生试验假阳性,根据普遍认可的分类方案,这会导致将这些培养物鉴定为大肠杆菌。考虑到这一特性,作者从实验室分离的22株培养物中又额外分离出20株典型的伤寒沙门氏菌菌株。

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