Lesmana M, Tjaniadi P, Edman D, Lane E
US Naval Medical Research Unit No. 2, Jakarta Detachment, APO San Francisco 96356-5000.
Southeast Asian J Trop Med Public Health. 1990 Jun;21(2):203-6.
A study was conducted to test a coagglutination procedure for detection of Salmonella typhi in bone marrow cultures from suspected typhoid patients admitted to Friendship Hospital, Jakarta, Indonesia. The results of the coagglutination tests were compared to the results from standard cultural isolation and identification. Bone marrow aspirates (356) were cultured in oxgall medium and aliquots subcultured daily for 7 days while simultaneously testing for the presence of Salmonella group D and Vi antigens using coagglutination (COAG). S. typhi was isolated from 220 (62%) of the cultures and the D- and Vi-COAG tests were positive for those same cultures. The COAG test was also negative for 6 cultures containing S. paratyphi A. The COAG results were available within 10 minutes after 18 to 24 hours incubation of the primary cultures whereas the isolation and confirmed identification took 2 to 3 days longer. The COAG test is valuable as an aid to rapidly identify S. typhi in bone marrow-oxgall cultures.
开展了一项研究，以检测用于在印度尼西亚雅加达友谊医院收治的疑似伤寒患者的骨髓培养物中检测伤寒沙门氏菌的协同凝集程序。将协同凝集试验的结果与标准培养分离和鉴定的结果进行比较。将356份骨髓抽吸物在牛胆汁培养基中培养，并每天分取 aliquots 进行传代培养，持续7天，同时使用协同凝集（COAG）检测伤寒沙门氏菌D群和Vi抗原的存在。从220份（62%）培养物中分离出伤寒沙门氏菌，并且对于相同的培养物，D-和Vi-COAG试验呈阳性。对于6份含有甲型副伤寒沙门氏菌的培养物，COAG试验也呈阴性。在原代培养物孵育18至24小时后10分钟内即可获得COAG结果，而分离和确认鉴定则需要2至3天时间。COAG试验作为快速鉴定骨髓-牛胆汁培养物中伤寒沙门氏菌的辅助手段具有重要价值。
