Bergström P, Grankvist K, Holm S, Henriksson R
Department of Oncology, University Hospital, Umeå, Sweden.
Anticancer Res. 1991 May-Jun;11(3):1039-43.
The effect of concomitant treatment with different antibiotics on the cytotoxicity of epirubicin, bleomycin, estramustine and cisplatin was studied in vitro on fibroblasts (V79) and two cancer cell lines (colon cancer HT29 and lung cancer P31). The cell lines were propagated under standard tissue culture conditions and evaluated as the number of surviving cell clones in comparison to untreated controls. Fifteen commonly used antibiotics were tested and thirteen of these were found to modify the cytotoxic effect in one or several of the combinations tested. One antibiotic agent could affect the toxicity of different cytostatics in opposite directions and there were marked differences between the cell lines tested. Only in one of the situations, the combination of bleomycin and ceftazidim, did the antibiotic cause opposite effects on the toxicity of a cytostatic when comparing fibroblasts and carcinoma cells. A most impressive observation was the pronounced increase in cisplatin-induced cytotoxicity by amphotericin B. In conclusion, the results suggest that antibiotics can interact with the cytotoxicity of antitumoral drugs but that the feature of this interaction is seemingly an erratic phenomenon. Further studies are certainly justified, especially regarding the effects of amphotericin B and its mechanisms in enhancing the cytotoxicity of cisplatin.
在体外对成纤维细胞（V79）以及两种癌细胞系（结肠癌HT29和肺癌P31）研究了不同抗生素联合治疗对表柔比星、博来霉素、雌莫司汀和顺铂细胞毒性的影响。细胞系在标准组织培养条件下增殖，并与未处理的对照相比，以存活细胞克隆数进行评估。测试了15种常用抗生素，其中13种被发现在一种或几种测试组合中改变了细胞毒性作用。一种抗生素可对不同的细胞抑制剂毒性产生相反的影响，并且在测试的细胞系之间存在显著差异。仅在一种情况下，即博来霉素与头孢他啶的组合中，当比较成纤维细胞和癌细胞时，抗生素对一种细胞抑制剂的毒性产生了相反的影响。一个最引人注目的观察结果是两性霉素B显著增加了顺铂诱导的细胞毒性。总之，结果表明抗生素可与抗肿瘤药物的细胞毒性相互作用，但这种相互作用的特征似乎是一种不稳定的现象。进一步的研究当然是合理的，特别是关于两性霉素B的作用及其增强顺铂细胞毒性的机制。
