围产期奶牛乳腺分泌物中的细胞因子活性

Sordillo L M, Redmond M J, Campos M, Warren L, Babiuk L A

Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon.

Can J Vet Res. 1991 Jul;55(3):298-301.

The presence of cytokine activity in periparturient bovine mammary secretions was evaluated. Mammary secretions were modified for use in biological assays for interleukin-2 (IL-2) like and antiviral activity. The level of IL-2 like activity in mammary gland secretions was lower during the last week of gestation when compared to levels detected approximately two weeks prepartum. Antiviral titers gradually increased as parturition approached. Results from Western blots indicated that the antiviral activity observed in prepartum secretions may be due to tumor necrosis factor (TNF). Interferons (IFN) were not detected in the colostrum samples.

对围产期奶牛乳腺分泌物中的细胞因子活性进行了评估。对乳腺分泌物进行了改良，用于白细胞介素-2（IL-2）样和抗病毒活性的生物学检测。与产前约两周检测到的水平相比，妊娠最后一周乳腺分泌物中IL-2样活性水平较低。随着分娩临近，抗病毒滴度逐渐升高。蛋白质印迹法结果表明，产前分泌物中观察到的抗病毒活性可能归因于肿瘤坏死因子（TNF）。初乳样本中未检测到干扰素（IFN）。