Karlidag Turgut, Cobanoglu Bengu, Keles Erol, Alpay Hayrettin Cengiz, Ozercan Ibrahim, Kaygusuz Irfan, Yalcin Sinasi, Sakallioglu Oner
Department of Otorhinolaryngology, Medical Faculty, Firat University, Elazig, Turkey.
Am J Otolaryngol. 2007 Jan-Feb;28(1):31-6. doi: 10.1016/j.amjoto.2006.06.008.
The purpose of this study is to investigate the expression of Bax, p53, and p27/kip1 in patients with papillary thyroid carcinoma (PTC) with or without cervical nodal metastasis and to determine the role of immunohistochemical markers for prediction of neck metastasis in these patients.
A retrospective review from 1998 to 2004 at the Department of Otorhinolaryngology, Firat University, Elazig, Turkey, was performed in the records of 28 patients with nonmetastasizing (n = 15) and metastasizing (n = 13) PTC. The immunoreactivity for Bax, p53, and p27/kip1 protein in all tissue specimens were assessed.
p27 expression in the nonmetastasizing PTC was lower than normal thyroid tissue (P < .01) and higher than metastasizing PTC (P < .01). p53 immunoreactivity was present, faint (grade 1) staining in a small number of cells in 2 nonmetastasizing PTCs. In 13 nonmetastasizing PTCs and all of metastasizing PTCs, normal thyroid tissue had no immunoreactivity for p53. There was no statistically significant difference among all groups' immunoreactivity for p53. Expression of Bax in patients with PTC was more than normal thyroid tissue (P < .01). However, expression of Bax in the patients with nonmetastasizing and metastasizing PTC was similar.
The immunohistochemical evaluation of p27 expression in patients with PTC may be useful to predict for metastasis risk of the PTC to regional lymph nodes.
本研究旨在调查有无颈部淋巴结转移的甲状腺乳头状癌（PTC）患者中Bax、p53和p27/kip1的表达情况，并确定免疫组化标志物在预测这些患者颈部转移中的作用。
对1998年至2004年土耳其埃拉泽菲拉特大学耳鼻喉科28例非转移性（n = 15）和转移性（n = 13）PTC患者的记录进行回顾性分析。评估所有组织标本中Bax、p53和p27/kip1蛋白的免疫反应性。
非转移性PTC中p27的表达低于正常甲状腺组织（P <.01），高于转移性PTC（P <.01）。在2例非转移性PTC中，少数细胞存在p53免疫反应性，呈弱阳性（1级）染色。在13例非转移性PTC和所有转移性PTC中，正常甲状腺组织对p53无免疫反应性。所有组对p53的免疫反应性无统计学显著差异。PTC患者中Bax的表达高于正常甲状腺组织（P <.01）。然而，非转移性和转移性PTC患者中Bax的表达相似。
对PTC患者p27表达进行免疫组化评估可能有助于预测PTC转移至区域淋巴结的风险。
