Millon Stacy R, Roldan-Perez Katherine M, Riching Kristin M, Palmer Gregory M, Ramanujam Nirmala
Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin 53706, USA.
Lasers Surg Med. 2006 Dec;38(10):920-7. doi: 10.1002/lsm.20451.
Optical clearing agents (OCAs) have previously been shown to increase depth penetration within turbid tissue ex vivo. This paper quantifies tissue optical properties of the hamster cheek pouch model in order to provide a means to assess the effect of OCAs quantitatively in vivo.
STUDY DESIGN/MATERIALS AND METHODS: Diffuse reflectance spectra were obtained from both cheeks of 12 hamsters before and after immersion in dimethyl sulfoxide (DMSO), glycerol or a phosphate buffer saline (PBS) control for 20 minutes. A Monte Carlo model was then utilized to derive the wavelength dependent reduced scattering and absorption coefficients.
DMSO caused a statistically significant decrease in the absorption and reduced scattering coefficients derived by the model. Glycerol caused a statistically significant increase in the wavelength dependent absorption coefficient, but no statistically significant changes in the reduced scattering coefficient.
DMSO and glycerol act upon tissues differently as reflected by the tissue optical properties, implying that not all OCAs are equally effective in optically clearing tissues.
光学透明剂（OCAs）此前已被证明可在体外增加对浑浊组织的深度穿透。本文对仓鼠颊囊模型的组织光学特性进行量化，以便提供一种在体内定量评估光学透明剂效果的方法。
研究设计/材料与方法：在12只仓鼠的双颊浸入二甲基亚砜（DMSO）、甘油或磷酸盐缓冲盐水（PBS）对照20分钟前后，获取其漫反射光谱。然后利用蒙特卡罗模型得出波长依赖性的约化散射系数和吸收系数。
DMSO使模型得出的吸收系数和约化散射系数出现统计学显著下降。甘油使波长依赖性吸收系数出现统计学显著增加，但约化散射系数无统计学显著变化。
如组织光学特性所反映的，DMSO和甘油对组织的作用不同，这意味着并非所有光学透明剂在光学清除组织方面都同样有效。
