Nakamura S, Takeda Y, Kanno M, Yoshida T, Ohtake S, Kobayashi K, Okabe Y, Matsuda T
Third Department of Medicine, Kanazawa University School of Medicine, Ishikawa, Japan.
Oncology. 1991;48(4):285-9. doi: 10.1159/000226944.
To investigate the proliferative characteristics of human bone marrow leukemic cells in vivo, we developed a new method using bromodeoxyuridine (BrdU) and its monoclonal antibody. Thirty minutes after a bolus BrdU infusion, a lst bone marrow aspiration (BMP) was performed to calculate the BrdU labeling index (BrdU-LI). Then, a 150-min continuous infusion of BrdU was started. Immediately after the infusion, a 2nd BMP was done to analyze the increasing rate of the BrdU-LI for 3 h. Each smear was stained by an immunohistochemical method. The BrdU-LI, the duration of S phase (Ts) and the cell cycle time (Tc) of leukemic cells from 13 previously untreated patients with acute leukemias were estimated. A mean value of 7.2% for BrdU-LI was obtained. Furthermore, mean values of 9.7 h and 152.5 h for Ts and Tc respectively were calculated. This method may prove to be useful in the in vivo cell cycle analysis of leukemias and other malignancies.
为研究人骨髓白血病细胞在体内的增殖特性，我们开发了一种使用溴脱氧尿苷（BrdU）及其单克隆抗体的新方法。在静脉推注BrdU 30分钟后，进行首次骨髓穿刺（BMP）以计算BrdU标记指数（BrdU-LI）。然后，开始连续150分钟输注BrdU。输注后立即进行第二次BMP，以分析BrdU-LI在3小时内的增加率。每张涂片采用免疫组织化学方法染色。对13例未经治疗的急性白血病患者的白血病细胞的BrdU-LI、S期持续时间（Ts）和细胞周期时间（Tc）进行了评估。获得的BrdU-LI平均值为7.2%。此外，计算出Ts和Tc的平均值分别为9.7小时和152.5小时。该方法可能被证明对白血病和其他恶性肿瘤的体内细胞周期分析有用。
