Perkins R Serene, Sahm Katherine, Marando Cindy, Dickson-Witmer Diana, Pahnke Gregory R, Mitchell Mark, Petrelli Nicholas J, Berkowitz Irving M, Soteropoulos Patricia, Aris Virginie M, Dunn Stephen P, Krueger Leslie J
Molecular Genetics, Cellular and Tissue Transplantation, Nemours Biomedical Research, Rockland Road, Wilmington, DE 19803, USA.
Breast Cancer Res. 2006;8(6):R70. doi: 10.1186/bcr1627.
Epstein-Barr virus (EBV) is present in over 90% of the world's population. This infection is considered benign, even though in limited cases EBV is associated with infectious and neoplastic conditions. Over the past decade, the EBV association with breast cancer has been constantly debated. Adding to this clinical and biological uncertainty, different techniques gave contradictory results for the presence of EBV in breast carcinoma specimens. In this study, minor groove binding (MGB)-TaqMan real time PCR was used to detect the presence of EBV DNA in both peripheral blood and tumor samples of selected patients.
Peripheral blood and breast carcinoma specimens from 24 patients were collected. DNA was extracted and then amplified by MGB-TaqMan real time PCR.
Of 24 breast tumor specimens, 11 (46%) were positive for EBV DNA. Of these 11 breast tumor specimens, 7 (64%) were also positive for EBV DNA in the peripheral blood, while 4 (36%) were positive for EBV DNA in the tumor, but negative in the blood.
EBV was found at extremely low levels, with a mean of 0.00004 EBV genomes per cell (range 0.00014 to 0.00001 EBV genomes per cell). Furthermore, our finding of the presence of EBV in the tumor specimens coupled to the absence of detection of EBV genomic DNA in the peripheral blood is consistent with the epithelial nature of the virus. Because of the low levels of viral DNA in tumor tissue, further studies are needed to assess the biological input of EBV in breast cancer.
爱泼斯坦-巴尔病毒（EBV）存在于全球90%以上的人口中。这种感染被认为是良性的，尽管在少数情况下EBV与感染性和肿瘤性疾病有关。在过去十年中，EBV与乳腺癌的关联一直存在争议。除了这种临床和生物学上的不确定性外，不同技术对乳腺癌标本中EBV的存在给出了相互矛盾的结果。在本研究中，使用小沟结合（MGB）-TaqMan实时PCR检测选定患者外周血和肿瘤样本中EBV DNA的存在。
收集24例患者的外周血和乳腺癌标本。提取DNA，然后通过MGB-TaqMan实时PCR进行扩增。
在24例乳腺肿瘤标本中，11例（46%）EBV DNA呈阳性。在这11例乳腺肿瘤标本中，7例（64%）外周血中EBV DNA也呈阳性，而4例（36%）肿瘤中EBV DNA呈阳性，但血液中呈阴性。
发现EBV水平极低，平均每个细胞有0.00004个EBV基因组（范围为每个细胞0.00014至0.00001个EBV基因组）。此外，我们在肿瘤标本中发现EBV存在而在外周血中未检测到EBV基因组DNA的结果与该病毒的上皮性质一致。由于肿瘤组织中病毒DNA水平较低，需要进一步研究来评估EBV在乳腺癌中的生物学作用。
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