Golrokh Mofrad Morvarid, Kazeminezhad Behrang, Faghihloo Ebrahim
Human Viral Vaccine Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Department of Pathology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Asian Pac J Cancer Prev. 2020 Jan 1;21(1):133-137. doi: 10.31557/APJCP.2020.21.1.133.
Breast cancer (BC) is the most common malignancy affecting females worldwide. Various risk factors play a role in the developing of BC. Infectious agents like viruses have been proposed for this cancer and Epstein-Barr virus (EBV) is a widely researched candidate virus. This study detects the presence of EBV-DNA in breast cancer patients.
The study was conducted on 59 formalin-fixed paraffin-embedded (FFPE) tissue blocks samples of women with breast carcinoma and 11 non-neoplastic breast controls. The DNA was extracted for all the samples. Then detection of EBNA1 EBV was done by polymerase chain reaction (PCR).
EBV was detected in 6.7% (4/59) of patients while all breast control samples were negative. All patients with positive EBV-DNA were high tumor grades (II, and III). Also, they had a low level of educations.
According to our findings, it can be suggested that EBV may have a potential role in breast cancer development. However, this study provides substantial but not conclusive evidence for the involvement of viruses in BC disease development. Therefore, future investigations are needed to elucidate the exact role of EBV in breast cancer.
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乳腺癌（BC）是全球影响女性的最常见恶性肿瘤。多种风险因素在乳腺癌的发生中起作用。病毒等感染因子被认为与这种癌症有关，而爱泼斯坦-巴尔病毒（EBV）是一种被广泛研究的候选病毒。本研究检测乳腺癌患者中EBV-DNA的存在情况。
对59例乳腺癌女性的福尔马林固定石蜡包埋（FFPE）组织块样本和11例非肿瘤性乳腺对照进行研究。提取所有样本的DNA。然后通过聚合酶链反应（PCR）检测EBNA1 EBV。
6.7%（4/59）的患者检测到EBV，而所有乳腺对照样本均为阴性。所有EBV-DNA阳性的患者肿瘤分级都很高（II级和III级）。此外，他们的教育程度较低。
根据我们的研究结果，可以认为EBV可能在乳腺癌发展中起潜在作用。然而，本研究为病毒参与BC疾病发展提供了大量但非决定性的证据。因此，需要进一步的研究来阐明EBV在乳腺癌中的确切作用。
