Dowran Razieh, Joharinia Negar, Safaei Akbar, Bakhtiyarizadeh Sahar, Alidadi Soleimani Abootaleb, Alizadeh Rasool, Mir-Shiri Sara, Sarvari Jamal
Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
BMC Res Notes. 2019 Mar 25;12(1):171. doi: 10.1186/s13104-019-4178-3.
The most common cancer amongst women is breast cancer. Reports on the role of EBV, BKV, and JCV in the development of breast cancer are controversial. Hence, the aim of this study was to determine the frequency of EBV, BKV, and JCV in malignant breast tumors in comparison with benign ones.
A total of 300 breast biopsy tissues were included, of which 150 were malignant and 150 benign. After deparaffinization, tissues were subjected to DNA extraction. β-globin gene was amplified by PCR to evaluate the quality of extracted DNA. In house PCRs assay was performed to detect EBV, JCV, and BKV genome fragment. The mean age of malignant and benign groups was 45.0 ± 9.4 and 35.2 ± 12.1 years old. Out of 150 malignant samples, 146 were ductal, two lobular and two samples both invasive ductal and lobular carcinoma. In the benign group, 96, 52 and two samples were fibroadenoma, fibrocystic, and adenosis types, respectively. Genomic DNA fragment of EBV, BKV, and JCV was not found in any of the malignant and benign breast tissues.
According to our finding, there is the possibility that EBV, BKV, and JCV are not involved in breast cancer pathogenesis.
女性中最常见的癌症是乳腺癌。关于EB病毒（EBV）、BK病毒（BKV）和JC病毒（JCV）在乳腺癌发生发展中的作用的报道存在争议。因此，本研究的目的是确定恶性乳腺肿瘤与良性乳腺肿瘤中EBV、BKV和JCV的感染频率。
共纳入300份乳腺活检组织，其中150份为恶性，150份为良性。脱蜡后，对组织进行DNA提取。通过聚合酶链反应（PCR）扩增β-珠蛋白基因以评估提取DNA的质量。采用自制的PCR检测法检测EBV、JCV和BKV基因组片段。恶性组和良性组的平均年龄分别为45.0±9.4岁和35.2±12.1岁。在150份恶性样本中，146份为导管癌，2份为小叶癌，2份为浸润性导管癌和小叶癌。在良性组中，分别有96份、52份和2份样本为纤维腺瘤、纤维囊性变和腺病类型。在任何恶性和良性乳腺组织中均未发现EBV、BKV和JCV的基因组DNA片段。
根据我们的研究结果，EBV、BKV和JCV有可能不参与乳腺癌的发病机制。
