育龄女性叶酸和维生素B12摄入量评估的验证：三联法
Validation of the assessment of folate and vitamin B12 intake in women of reproductive age: the method of triads.
作者信息
Verkleij-Hagoort A C, de Vries J H M, Stegers M P G, Lindemans J, Ursem N T C, Steegers-Theunissen R P M
机构信息
Department of Obstetrics and Gynaecology, Division of Obstetrics and Prenatal Medicine, Erasmus MC, University Medical Centre, Rotterdam, The Netherlands.
出版信息
Eur J Clin Nutr. 2007 May;61(5):610-5. doi: 10.1038/sj.ejcn.1602581. Epub 2006 Dec 13.
OBJECTIVE
To validate the folate and vitamin B12 intakes estimated by a food-frequency questionnaire (FFQ) designed to be used in a case-control study on the association between maternal dietary intake and the risk of having a child with a congenital heart defect.
DESIGN AND SUBJECTS
The FFQ was filled out by 53 women of reproductive age. Immediately thereafter, blood samples were taken to determine serum folate, red blood cell (RBC) folate and serum vitamin B12 concentrations. Subsequently, three dietary 24-h recalls (24HR) were completed during a period of three successive weeks and used as a reference method. The recalls comprised two weekdays and one weekend day. Using the method of triads, validity coefficients were calculated by comparing nutrient intakes derived from the FFQ and 24HR with the corresponding nutritional biomarkers in blood. The validity coefficient is the correlation between the dietary intake reported by the FFQ and the unknown 'true' dietary intake.
RESULTS
The comparison of B-vitamin intakes reported by the FFQ and the mean of the 24HR revealed deattenuated correlation coefficients of 0.98 for folate and 0.66 for vitamin B12. The correlation coefficients between the B-vitamin intakes estimated by the FFQ and concentrations of serum folate, RBC folate and serum vitamin B12 were 0.20, 0.28 and 0.21, respectively. The validity coefficients for serum folate, RBC folate and serum vitamin B12 were 0.94, 0.75 and 1.00, respectively. The estimated folate and vitamin B12 intakes were comparable with the results of the most recent Dutch food consumption survey.
CONCLUSIONS
The adapted FFQ is a reliable tool to estimate the dietary intake of energy, macronutrients, folate and vitamin B12 in women of reproductive age. Therefore, this FFQ is suitable for the investigation of nutrient-disease associations in future.
SPONSORSHIP
Funding was provided by the Netherlands Heart Foundation (Grant 2002.B027).
目的
验证一份食物频率问卷（FFQ）所估算的叶酸和维生素B12摄入量，该问卷旨在用于一项关于母亲饮食摄入量与孩子患先天性心脏病风险之间关联的病例对照研究。
设计与研究对象
53名育龄妇女填写了FFQ。随后立即采集血样以测定血清叶酸、红细胞（RBC）叶酸和血清维生素B12浓度。之后，在连续三周的时间里完成了三次24小时饮食回顾（24HR），并将其用作参考方法。这些回顾包括两个工作日和一个周末日。采用三联法，通过比较FFQ和24HR得出的营养素摄入量与血液中相应的营养生物标志物，计算有效性系数。有效性系数是FFQ报告的饮食摄入量与未知的“真实”饮食摄入量之间的相关性。
结果
FFQ报告的B族维生素摄入量与24HR均值的比较显示，叶酸的去衰减相关系数为0.98，维生素B12为0.66。FFQ估算的B族维生素摄入量与血清叶酸、RBC叶酸和血清维生素B12浓度之间的相关系数分别为0.20、0.28和0.21。血清叶酸、RBC叶酸和血清维生素B12的有效性系数分别为0.94、0.75和1.00。估算的叶酸和维生素B12摄入量与荷兰最近的食物消费调查结果相当。
结论
经过调整的FFQ是估算育龄妇女能量、常量营养素、叶酸和维生素B12饮食摄入量的可靠工具。因此，这份FFQ适用于未来营养与疾病关联的研究。
资助情况
由荷兰心脏基金会提供资金（资助编号2002.B027）。
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