一种基于纳米催化剂的蛋白质检测方法：使用金纳米颗粒标记物催化还原对硝基苯酚的无DNA超灵敏电化学检测。

A nanocatalyst-based assay for proteins: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels.

作者信息

Das Jagotamoy, Aziz Md Abdul, Yang Haesik

机构信息

Department of Chemistry, Pusan National University, Busan 609-735, Korea.

出版信息

J Am Chem Soc. 2006 Dec 20;128(50):16022-3. doi: 10.1021/ja0672167.

DOI:10.1021/ja0672167

PMID:17165740

Abstract

This communication reports a nanocatalyst-based electrochemical assay for proteins. Ultrasensitive detection has been achieved by signal amplification combined with noise reduction: the signal is amplified both by the catalytic reduction of p-nitrophenol to p-aminophenol by gold-nanocatalyst labels and by the chemical reduction of p-quinone imine to p-aminophenol by NaBH4; the noise is reduced by employing an indium tin oxide electrode modified with a ferrocenyl-tethered dendrimer and a hydrophilic immunosensing layer.

摘要

本通讯报道了一种基于纳米催化剂的蛋白质电化学检测方法。通过信号放大与降噪相结合实现了超灵敏检测：信号通过金纳米催化剂标记将对硝基苯酚催化还原为对氨基苯酚以及硼氢化钠将对醌亚胺化学还原为对氨基苯酚来放大；通过使用修饰有二茂铁连接的树枝状大分子和亲水免疫传感层的氧化铟锡电极来降低噪声。