Lousch R N, Staats H, Oakes J E, Cohen G H, Eisenberg R J
Department of Microbiology/Immunology, University of South Alabama, Mobile 36688.
Invest Ophthalmol Vis Sci. 1991 Sep;32(10):2735-40.
Seven monoclonal antibodies (mAb) specific for defined discontinuous and continuous epitopes on glycoprotein D of herpes simplex virus type 1 (HSV-1) were surveyed for their capacity to protect against virus-induced corneal disease in a murine ocular infection model. A known amount of purified mAb was transferred passively to BALB/c mice 24 hr after topical infection with HSV-1 on their scarified corneas. At high doses (50-136 micrograms), all seven mAbs protected against the development of persistent necrotizing stromal keratitis. Significant protection was also observed at low doses (20 micrograms) with two mAbs to discontinuous epitopes and two mAbs to continuous epitopes. Selected high-dose mAbs also were able to reduce the severity of blepharitis. These results indicated that at least seven different antigenic sites on glycoprotein D can serve as targets for effective antibody therapy in the murine model of HSV-1 ocular infection.
在小鼠眼部感染模型中，对七种针对单纯疱疹病毒1型（HSV-1）糖蛋白D上特定不连续和连续表位的单克隆抗体（mAb）进行了研究，以考察它们预防病毒诱导的角膜疾病的能力。在经划痕处理的角膜上局部感染HSV-1 24小时后，将已知量的纯化单克隆抗体被动转移至BALB/c小鼠体内。在高剂量（50 - 136微克）时，所有七种单克隆抗体都能预防持续性坏死性基质角膜炎的发展。在低剂量（20微克）时，两种针对不连续表位的单克隆抗体和两种针对连续表位的单克隆抗体也观察到了显著的保护作用。选定的高剂量单克隆抗体还能够减轻睑缘炎的严重程度。这些结果表明，在HSV-1眼部感染的小鼠模型中，糖蛋白D上至少七个不同的抗原位点可作为有效抗体治疗的靶点。
