Ahuja Deepak K, Bachas Leonidas G, Bhattacharyya D
Department of Chemical and Materials Engineering, University of Kentucky, 177 Anderson Hall, Lexington, KY 40506-0046, United States.
Chemosphere. 2007 Feb;66(11):2193-200. doi: 10.1016/j.chemosphere.2006.08.035. Epub 2006 Dec 12.
Glucose oxidase is a well-known enzyme that catalyzes the oxidation of beta-D-glucose to produce gluconic acid and hydrogen peroxide. Fenton reaction is a powerful oxidation technology used for the oxidation of groundwater pollutants. For the application of Fenton reaction in groundwater remediation, successful operation of Fenton reaction near neutral pH, and on-site generation of both H2O2 and chelate will be beneficial. The focus of this experimental study was to couple the glucose oxidation reaction with chelate-based Fenton reaction. The idea was to use the hydrogen peroxide and chelate gluconic acid generated during glucose oxidation for the dechlorination of 2,4,6-trichlorophenol (TCP) by Fenton reaction. The oxidation of glucose was achieved using the enzyme in free and immobilized forms. The rate of production of hydrogen peroxide was determined for each system, and was used to estimate the time required for complete consumption of glucose during the process, thus avoiding any traces of glucose in the Fenton reaction. In the case of free enzyme reaction, separation of the enzyme was achieved using an ultrafiltration membrane before initiating the Fenton reaction. The oxidation of TCP by Fenton reaction was performed at varying ratios of gluconic acid/Fe, and its effect on the decomposition of TCP and H2O2 was studied. TCP degradation was studied both in terms of parent compound degradation and free chloride generation.
葡萄糖氧化酶是一种著名的酶，它催化β-D-葡萄糖氧化生成葡萄糖酸和过氧化氢。芬顿反应是一种用于氧化地下水中污染物的强大氧化技术。对于芬顿反应在地下水修复中的应用，在接近中性pH值下成功运行芬顿反应以及现场生成H2O2和螯合剂将是有益的。本实验研究的重点是将葡萄糖氧化反应与基于螯合剂的芬顿反应相结合。其思路是利用葡萄糖氧化过程中产生的过氧化氢和螯合葡萄糖酸，通过芬顿反应对2,4,6-三氯苯酚（TCP）进行脱氯。葡萄糖的氧化通过游离形式和固定化形式的酶来实现。测定了每个系统中过氧化氢的产生速率，并用于估计该过程中葡萄糖完全消耗所需的时间，从而避免芬顿反应中残留任何葡萄糖。在游离酶反应的情况下，在启动芬顿反应之前，使用超滤膜实现酶的分离。通过芬顿反应氧化TCP时，改变葡萄糖酸/铁的比例，并研究其对TCP和H2O2分解的影响。从母体化合物降解和游离氯生成两方面研究了TCP的降解情况。
