蜜蜂毒液对脂多糖刺激的小胶质细胞一氧化氮和肿瘤坏死因子-α产生的影响。

Effect of honey bee venom on microglial cells nitric oxide and tumor necrosis factor-alpha production stimulated by LPS.

作者信息

Han SangMi, Lee KwangGill, Yeo JooHong, Kweon HaeYong, Woo SoonOk, Lee MyeongLyeol, Baek HaJu, Kim SunYeou, Park KwanKyu

机构信息

Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Suwon, South Korea.

出版信息

J Ethnopharmacol. 2007 Apr 20;111(1):176-81. doi: 10.1016/j.jep.2006.11.008. Epub 2006 Nov 15.

DOI:10.1016/j.jep.2006.11.008

PMID:17166679

Abstract

Abnormal activation of microglial cells has been implicated in various neurodegenerative diseases. Results showed that venom (KBV) produced and purified in Korea regulated lipopolysaccharides (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) in the murine microglia, BV-2 cell line. The production of proinflammatory cytokines, NO, and TNF-alpha was examined by LPS in BV-2 cell. The effect of KBV on the expression of inducible nitric oxide synthase (iNOS) and TNF-alpha was investigated by Western blot and RT-PCR in LPS-stimulated BV-2 cells. KBV suppressed the NO, iNOS, and TNF-alpha production, and decreased the levels of iNOS and TNF-alpha mRNA. These results suggest that KBV has anti-inflammatory properties that inhibit iNOS and TNF-alpha expression. KBV could be useful in inhibiting the production of inflammatory cytokine and NO production in neurodegenerative diseases. Further studies on the pharmacological aspects of the individual components of KBV are recommended.

摘要

小胶质细胞的异常激活与多种神经退行性疾病有关。结果表明，韩国生产和纯化的毒液（KBV）可调节小鼠小胶质细胞系BV-2中脂多糖（LPS）诱导的一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）。通过LPS检测BV-2细胞中促炎细胞因子、NO和TNF-α的产生。通过蛋白质印迹法和逆转录-聚合酶链反应（RT-PCR）研究KBV对LPS刺激的BV-2细胞中诱导型一氧化氮合酶（iNOS）和TNF-α表达的影响。KBV抑制了NO、iNOS和TNF-α的产生，并降低了iNOS和TNF-α mRNA的水平。这些结果表明，KBV具有抗炎特性，可抑制iNOS和TNF-α的表达。KBV可能有助于抑制神经退行性疾病中炎性细胞因子的产生和NO的产生。建议对KBV的各个成分进行进一步的药理学研究。