猴免疫缺陷病毒SIVmac nef基因的突变分析
Mutational analysis of the simian immunodeficiency virus SIVmac nef gene.
作者信息
Binninger D, Ennen J, Bonn D, Norley S G, Kurth R
机构信息
Paul-Ehrlich-Institut, Langen, Germany.
出版信息
J Virol. 1991 Oct;65(10):5237-43. doi: 10.1128/JVI.65.10.5237-5243.1991.
Abstract
We are using site-directed mutagenesis of single viral genes to identify and analyze the genetic determinants of human and simian immunodeficiency virus pathogenicity. In a first approach, we have constructed a series of simian immunodeficiency virus SIVmac nef mutants by partial deletion and insertions in the nef gene, as this gene is a candidate gene for the establishment and maintenance of latency. nef insertion mutants replicated faster than wild-type SIVmac, suggesting that the nef gene product acts as a negative factor for replication. Surface phenotyping revealed that cultures permanently infected with nef mutants exhibit an enhanced expression of viral proteins on the outer cell surface. We have analyzed the properties of the mutant viruses in cell culture and intend to use rapidly replicating mutants (putatively unable to undergo latency) as model vaccine viruses in the rhesus monkey.
摘要
我们正在利用单病毒基因的定点诱变来鉴定和分析人类及猿猴免疫缺陷病毒致病性的遗传决定因素。在第一种方法中，我们通过在nef基因中进行部分缺失和插入构建了一系列猿猴免疫缺陷病毒SIVmac nef突变体，因为该基因是潜伏期建立和维持的候选基因。nef插入突变体的复制速度比野生型SIVmac快，这表明nef基因产物作为复制的负性因子发挥作用。表面表型分析显示，被nef突变体永久感染的培养物在外细胞表面呈现出病毒蛋白表达增强的现象。我们已经在细胞培养中分析了突变病毒的特性，并打算将快速复制的突变体（推测无法进入潜伏期）用作恒河猴的模型疫苗病毒。
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