• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用保守的XbaI片段脉冲场凝胶电泳鉴定猪源沙门氏菌血清型

Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes.

作者信息

Gaul Stephen B, Wedel Stephanie, Erdman Matthew M, Harris D L, Harris Isabel Turney, Ferris Kathleen E, Hoffman Lorraine

机构信息

Department of Animal Science, Iowa State University, 11 Kildee Hall, Ames, IA 50011, USA.

出版信息

J Clin Microbiol. 2007 Feb;45(2):472-6. doi: 10.1128/JCM.00962-06. Epub 2006 Dec 13.

DOI:10.1128/JCM.00962-06
PMID:17166969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1829035/
Abstract

Swine Salmonella isolates (n=674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with XbaI. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using XbaI restriction provided a possible alternative method for screening and identifying swine Salmonella serotypes.

摘要

对来自美国和加拿大各地的674株猪源沙门氏菌分离株，采用XbaI进行脉冲场凝胶电泳（PFGE）分析。通过聚类分析对PFGE亚型进行分析，并与传统血清分型结果进行比较。分析表明血清型与PFGE亚型之间存在相关性。此外，在每种血清型特有的限制性酶切图谱中鉴定出了保守片段。使用XbaI酶切的PFGE为猪源沙门氏菌血清型的筛选和鉴定提供了一种可能的替代方法。

相似文献

1
Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes.利用保守的XbaI片段脉冲场凝胶电泳鉴定猪源沙门氏菌血清型
J Clin Microbiol. 2007 Feb;45(2):472-6. doi: 10.1128/JCM.00962-06. Epub 2006 Dec 13.
2
Comparison of pulsed field gel electrophoresis and repetitive sequence polymerase chain reaction as genotyping methods for detection of genetic diversity and inferring transmission of Salmonella.脉冲场凝胶电泳和重复序列聚合酶链反应作为检测沙门氏菌遗传多样性和推断传播的基因分型方法的比较
Vet Microbiol. 2004 Jun 3;100(3-4):205-17. doi: 10.1016/j.vetmic.2004.02.009.
3
Finding the sources of Korean Salmonella enterica serovar Enteritidis PT 4 isolates by pulsed-field gel electrophoresis.通过脉冲场凝胶电泳法寻找韩国肠炎沙门氏菌肠炎血清型PT4分离株的来源。
J Microbiol. 2005 Oct;43(5):424-9.
4
Identification of patterns of transmission of Salmonella within swine production systems using pulsed field gel electrophoresis (PFGE) and repetitive sequence polymerase chain reaction (REP-PCR): a quantitative analysis.使用脉冲场凝胶电泳（PFGE）和重复序列聚合酶链反应（REP-PCR）鉴定猪生产系统中沙门氏菌的传播模式：定量分析
Berl Munch Tierarztl Wochenschr. 2001 Sep-Oct;114(9-10):397-400.
5
Adapting to contour-clamped homogeneous electric field minichamber technology the PulseNet protocols to resolve XbaI-DNA fragments of Salmonella serotype Braenderup.将脉冲网络协议应用于轮廓夹钳均匀电场小室技术，以解析肠炎沙门氏菌布伦德里普血清型的XbaI - DNA片段。
Anal Biochem. 2009 May 15;388(2):339-41. doi: 10.1016/j.ab.2009.02.038. Epub 2009 Mar 4.
6
Unveiling contamination sources and dissemination routes of Salmonella sp. in pigs at a Portuguese slaughterhouse through macrorestriction profiling by pulsed-field gel electrophoresis.通过脉冲场凝胶电泳的宏观限制性酶切图谱分析揭示葡萄牙一家屠宰场猪体内沙门氏菌的污染源和传播途径。
Int J Food Microbiol. 2006 Jul 1;110(1):77-84. doi: 10.1016/j.ijfoodmicro.2006.01.046. Epub 2006 Jul 7.
7
Pulsed-field gel electrophoresis subtyping database for foodborne Salmonella enterica serotype discrimination.用于食源肠炎沙门氏菌血清型鉴别的脉冲场凝胶电泳分型数据库。
Foodborne Pathog Dis. 2007 Fall;4(3):293-303. doi: 10.1089/fpd.2007.0090.
8
Pulsed-field gel electrophoresis genotyping of Salmonella gallinarum and comparison with random amplified polymorphic DNA.鸡沙门氏菌的脉冲场凝胶电泳基因分型及与随机扩增多态性DNA的比较
Vet Microbiol. 2006 Jul 20;115(4):349-57. doi: 10.1016/j.vetmic.2006.02.019. Epub 2006 Apr 11.
9
Molecular characterization of Salmonella enterica Serotype Typhi isolates by pulsed-field gel electrophoresis in Hong Kong, 2000-2004.2000 - 2004年香港地区伤寒沙门氏菌血清型伤寒杆菌分离株的脉冲场凝胶电泳分子特征分析
Foodborne Pathog Dis. 2007 Spring;4(1):41-9. doi: 10.1089/fpd.2006.59.
10
A comparison of Salmonella serotypes isolated from New Zealand sea lions and feral pigs on the Auckland Islands by pulsed-field gel electrophoresis.通过脉冲场凝胶电泳对从奥克兰群岛的新西兰海狮和野猪中分离出的沙门氏菌血清型进行比较。
J Wildl Dis. 2004 Jul;40(3):566-70. doi: 10.7589/0090-3558-40.3.566.

引用本文的文献

1
Assessing the mechanisms of multi-drug resistant non-typhoidal Salmonella (NTS) serovars isolated from layer chicken farms in Nigeria.评估尼日利亚蛋鸡养殖场分离的多重耐药性非伤寒沙门氏菌（NTS）血清型的机制。
PLoS One. 2023 Sep 7;18(9):e0290754. doi: 10.1371/journal.pone.0290754. eCollection 2023.
2
Phenotypic and Genetic Comparison of a Plant-Internalized and an Animal-Isolated Choleraesuis Strain.一株植物内源性猪霍乱沙门氏菌菌株与一株动物分离的猪霍乱沙门氏菌菌株的表型和基因比较
Microorganisms. 2021 Jul 21;9(8):1554. doi: 10.3390/microorganisms9081554.
3
Assessment and Comparison of Molecular Subtyping and Characterization Methods for .用于……的分子亚型分型及表征方法的评估与比较
Front Microbiol. 2019 Jul 12;10:1591. doi: 10.3389/fmicb.2019.01591. eCollection 2019.
4
Prevalence, Serotyping, Molecular Typing, and Antimicrobial Resistance of Isolated From Conventional and Organic Retail Ground Poultry.从传统和有机零售碎禽肉中分离出的[病原体名称未给出]的流行率、血清分型、分子分型及抗菌药物耐药性
Front Microbiol. 2018 Nov 5;9:2653. doi: 10.3389/fmicb.2018.02653. eCollection 2018.
5
A novel procedure on next generation sequencing data analysis using text mining algorithm.一种使用文本挖掘算法进行下一代测序数据分析的新方法。
BMC Bioinformatics. 2016 May 13;17(1):213. doi: 10.1186/s12859-016-1075-9.
6
A composite model for subgroup identification and prediction via bicluster analysis.一种通过双聚类分析进行亚组识别和预测的复合模型。
PLoS One. 2014 Oct 27;9(10):e111318. doi: 10.1371/journal.pone.0111318. eCollection 2014.
7
Irrigation water quality and the benefits of implementing good agricultural practices during tomato (Lycopersicum esculentum) production.灌溉水质以及番茄（Lycopersicum esculentum）生产过程中实施良好农业实践的益处。
Environ Monit Assess. 2014 Jul;186(7):4323-30. doi: 10.1007/s10661-014-3701-1. Epub 2014 Mar 31.
8
Prevalence and characterization of Salmonella enterica from the feces of cattle, poultry, swine and hedgehogs in Burkina Faso and their comparison to human Salmonella isolates.布基纳法索牛、禽、猪和刺猬粪便中沙门氏菌的流行情况及特征分析及其与人源沙门氏菌分离株的比较。
BMC Microbiol. 2013 Nov 11;13:253. doi: 10.1186/1471-2180-13-253.
9
Comparison of typing methods with a new procedure based on sequence characterization for Salmonella serovar prediction.基于序列特征的新型沙门氏菌血清型预测方法与传统分型方法的比较。
J Clin Microbiol. 2013 Jun;51(6):1786-97. doi: 10.1128/JCM.03201-12. Epub 2013 Apr 3.
10
Meta-analysis of pulsed-field gel electrophoresis fingerprints based on a constructed Salmonella database.基于构建的沙门氏菌数据库的脉冲场凝胶电泳指纹图谱的荟萃分析。
PLoS One. 2013;8(3):e59224. doi: 10.1371/journal.pone.0059224. Epub 2013 Mar 14.

本文引用的文献

1
Multilocus sequence typing lacks the discriminatory ability of pulsed-field gel electrophoresis for typing Salmonella enterica serovar Typhimurium.多位点序列分型缺乏脉冲场凝胶电泳对鼠伤寒沙门氏菌血清型分型的鉴别能力。
J Clin Microbiol. 2005 May;43(5):2215-9. doi: 10.1128/JCM.43.5.2215-2219.2005.
2
Use of pulsed-field gel electrophoresis to characterize the heterogeneity and clonality of salmonella isolates obtained from the carcasses and feces of swine at slaughter.使用脉冲场凝胶电泳来表征从屠宰猪的胴体和粪便中分离出的沙门氏菌菌株的异质性和克隆性。
Appl Environ Microbiol. 2003 Jul;69(7):4177-82. doi: 10.1128/AEM.69.7.4177-4182.2003.
3
Combined use of two genetic fingerprinting methods, pulsed-field gel electrophoresis and ribotyping, for characterization of Escherichia coli O157 isolates from food animals, retail meats, and cases of human disease.联合使用脉冲场凝胶电泳和核糖体分型这两种基因指纹图谱方法，对来自食用动物、零售肉类和人类疾病病例的大肠杆菌O157分离株进行特征分析。
J Clin Microbiol. 2002 Aug;40(8):2806-12. doi: 10.1128/JCM.40.8.2806-2812.2002.
4
Profile of Salmonella enterica subsp. enterica (subspecies I) serotype 4,5,12:i:- strains causing food-borne infections in New York City.肠炎沙门氏菌亚种肠炎亚种（I亚种）血清型4,5,12:i:-菌株在纽约市引起食源性感染的概况。
J Clin Microbiol. 2002 Jun;40(6):1924-9. doi: 10.1128/JCM.40.6.1924-1929.2002.
5
Multilocus sequence typing for characterization of clinical and environmental salmonella strains.用于临床和环境中沙门氏菌菌株特征分析的多位点序列分型
J Clin Microbiol. 2002 May;40(5):1626-35. doi: 10.1128/JCM.40.5.1626-1635.2002.
6
Molecular typing of Salmonella serotypes prevalent in animals in England: assessment of methodology.英格兰动物中流行的沙门氏菌血清型的分子分型：方法学评估。
J Clin Microbiol. 2001 Oct;39(10):3609-16. doi: 10.1128/JCM.39.10.3609-3616.2001.
7
Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica typhimurium DT104.鼠伤寒沙门氏菌DT104中多重耐药性的分子特征、传播与进化
Vet Res. 2001 May-Aug;32(3-4):301-10. doi: 10.1051/vetres:2001126.
8
PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States.脉冲网：美国食源性细菌疾病监测的分子分型网络。
Emerg Infect Dis. 2001 May-Jun;7(3):382-9. doi: 10.3201/eid0703.010303.
9
Use of molecular subtyping in surveillance for Salmonella enterica serotype typhimurium.分子分型在肠炎沙门氏菌鼠伤寒血清型监测中的应用。
N Engl J Med. 2001 Jan 18;344(3):189-95. doi: 10.1056/NEJM200101183440305.
10
Suitability of PCR fingerprinting, infrequent-restriction-site PCR, and pulsed-field gel electrophoresis, combined with computerized gel analysis, in library typing of Salmonella enterica serovar enteritidis.聚合酶链反应（PCR）指纹图谱、低频限制性酶切位点PCR以及脉冲场凝胶电泳结合计算机凝胶分析在肠炎沙门氏菌血清型肠炎亚种文库分型中的适用性。
Appl Environ Microbiol. 2000 Dec;66(12):5273-81. doi: 10.1128/AEM.66.12.5273-5281.2000.