用于临床和环境中沙门氏菌菌株特征分析的多位点序列分型
Multilocus sequence typing for characterization of clinical and environmental salmonella strains.
作者信息
Kotetishvili Mamuka, Stine O Colin, Kreger Arnold, Morris J Glenn, Sulakvelidze Alexander
机构信息
Division of Molecular Epidemiology, Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, 10 South Pine Street, Baltimore, MD 21201, USA.
出版信息
J Clin Microbiol. 2002 May;40(5):1626-35. doi: 10.1128/JCM.40.5.1626-1635.2002.
Abstract
Multilocus sequence typing (MLST) based on the 16S RNA, pduF, glnA, and manB genes was developed for Salmonella, and its discriminatory ability was compared to those of pulsed-field gel electrophoresis (PFGE) and serotyping. PFGE differentiated several strains undifferentiable by serotyping, and 78 distinct PFGE types were identified among 231 Salmonella isolates grouped into 22 serotypes and 12 strains of undetermined serotype. The strains of several PFGE types were further differentiated by MLST, which suggests that the discriminatory ability of MLST for the typing of Salmonella is better than that of serotyping and/or PFGE typing. manB-based sequence typing identified two distinct genetic clusters containing 32 of 54 (59%) clinical isolates whose manB gene sequences were analyzed. The G+C contents and Splitstree analysis of the manB, glnA, and pduF genes of Salmonella indicated that the genes differ in their evolutionary origins and that recombination played a significant role in their evolution.
摘要
基于16S RNA、pduF、glnA和manB基因的多位点序列分型（MLST）技术被应用于沙门氏菌，并将其鉴别能力与脉冲场凝胶电泳（PFGE）和血清分型的鉴别能力进行了比较。PFGE区分了几种血清分型无法区分的菌株，在231株分为22种血清型和12株血清型未确定的沙门氏菌分离株中，鉴定出78种不同的PFGE型。几种PFGE型的菌株通过MLST进一步区分，这表明MLST对沙门氏菌分型的鉴别能力优于血清分型和/或PFGE分型。基于manB的序列分型鉴定出两个不同的基因簇，包含54株临床分离株中的32株（59%），这些分离株的manB基因序列进行了分析。沙门氏菌manB、glnA和pduF基因的G+C含量及分裂树分析表明，这些基因的进化起源不同，重组在其进化中起了重要作用。
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