Tn抗原在T淋巴细胞系Jurkat细胞上的表达。

Expression of the Tn antigen on T-lymphoid cell line Jurkat.

作者信息

Nakada H, Inoue M, Tanaka N, Numata Y, Kitagawa H, Fukui S, Yamashina I

机构信息

Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Sep 16;179(2):762-7. doi: 10.1016/0006-291x(91)91882-d.

DOI:10.1016/0006-291x(91)91882-d

PMID:1716888

Abstract

Expression of the Tn antigen on a T-lymphoid cell line, Jurkat, was investigated using an anti-Tn monoclonal antibody, MLS 128. Immunoprecipitation or immunoaffinity chromatography of a lysate of Jurkat cells led to the isolation of a 120 kDa glycoprotein carrying the Tn antigen. This glycoprotein and leukosialin (CD43) were indistinguishable on SDS-PAGE and as to immunoreactivity with MLS 128. Leukosialin from an erythroid cell line, K562, exhibited no reactivity with MLS 128 despite that this leukosialin has several GalNAc alpha-Ser(Thr) structures. Pulse-chase experiments with the Jurkat leukosialin showed that newly synthesized leukosialin acquired the antigenecity after a lag of about 30 min, whereas incorporation of GalNAc into the leukosialin occurred earlier. These results indicate that the Tn antigen is expressed on leukosialin and that its epitopic structure is more complex than GalNAc alpha-Ser(Thr).

摘要

使用抗Tn单克隆抗体MLS 128研究了Tn抗原在T淋巴细胞系Jurkat上的表达。对Jurkat细胞裂解物进行免疫沉淀或免疫亲和层析，分离出了一种携带Tn抗原的120 kDa糖蛋白。该糖蛋白与白细胞涎酸蛋白（CD43）在SDS-PAGE上以及与MLS 128的免疫反应性方面无法区分。来自红系细胞系K562的白细胞涎酸蛋白与MLS 128无反应，尽管该白细胞涎酸蛋白有多个GalNAcα-Ser（Thr）结构。对Jurkat白细胞涎酸蛋白进行脉冲追踪实验表明，新合成的白细胞涎酸蛋白在约30分钟的延迟后获得抗原性，而GalNAc掺入白细胞涎酸蛋白的过程发生得更早。这些结果表明Tn抗原在白细胞涎酸蛋白上表达，且其表位结构比GalNAcα-Ser（Thr）更复杂。