Ribeiro D A, Marques M E A, Salvadori D M F
Department of Health Sciences, Federal University of Sao Paulo, UNIFESP, SP, Brazil.
J Oral Rehabil. 2006 Dec;33(12):912-7. doi: 10.1111/j.1365-2842.2006.01658.x.
Glass-ionomer cements are widely used in dentistry as restorative materials and adhesives for composite restorations. A number of genotoxicity studies have been conducted using these materials with results conflicting so far. Thus, the approach was aimed to look at the genotoxic and cytotoxic potential of three different glass-ionomer cements available commercially (Ketac Cem, Ketac Molar and Vitrebond) by the single cell gel (comet) assay and trypan blue exclusion test, respectively. For this, such materials were exposed to mouse lymphoma cells in vitro for 1 h at 37 degrees C. Data were assessed by Kruskall-Wallis non-parametric test. The results showed that all powders assayed did not show genotoxic effects. On the other hand, the liquid from Vitrebond at 0.1% dilution caused an increase of DNA injury. Significant statistically differences (P < 0.05) in cytotoxicity provoked by all powders tested were observed for exposure at 1,000 micro g mL(-1) concentration and 100 micro g mL(-1) for Ketac Molar. With respect to liquids of glass-ionomer cements evaluated, the major toxic effect on cell viability was produced at 1%, beginning at the dilution of 0.5% for Vitrebond. Taken together, these results support the notion that some components of glass-ionomer cements show both genotoxic and cytotoxic effects in higher concentrations.
玻璃离子水门汀作为修复材料和复合修复体的黏合剂在牙科领域被广泛应用。目前已经开展了多项关于这些材料的遗传毒性研究，但结果相互矛盾。因此，本研究旨在分别通过单细胞凝胶（彗星）试验和台盼蓝排斥试验，研究三种市售不同玻璃离子水门汀（Ketac Cem、Ketac Molar和Vitrebond）的遗传毒性和细胞毒性潜力。为此，将这些材料在37℃下体外暴露于小鼠淋巴瘤细胞1小时。数据通过克鲁斯卡尔 - 沃利斯非参数检验进行评估。结果表明，所有检测的粉末均未显示出遗传毒性作用。另一方面，Vitrebond稀释至0.1%的液体导致DNA损伤增加。在1000μg/mL浓度下，所有测试粉末引发的细胞毒性存在显著统计学差异（P < 0.05），Ketac Molar在100μg/mL时也有差异。对于所评估的玻璃离子水门汀液体，对细胞活力的主要毒性作用在1%时产生，Vitrebond从0.5%的稀释度开始。综上所述，这些结果支持以下观点：玻璃离子水门汀的某些成分在较高浓度下表现出遗传毒性和细胞毒性作用。
