Ribeiro Daniel Araki, Sugui Marina Mariko, Matsumoto Mariza Akemi, Duarte Marco Antonio Húngaro, Marques Mariangela Esther Alencar, Salvadori Daisy Maria Favero
Department of Pathology, Botucatu Medical School, Botucatu, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Feb;101(2):258-61. doi: 10.1016/j.tripleo.2005.02.080. Epub 2005 Oct 14.
Recently, mineral trioxide aggregate (MTA) and Portland cement have been used in dentistry as root-end-filling materials. However, the reported results concerning the biocompatibility of these materials are inconsistent. The goal of this study was to examine the genotoxicity and cytotoxicity of MTA and Portland cements in vitro by the single-cell gel (comet) assay and trypan blue exclusion test.
Chinese hamster ovary (CHO) cells were exposed to MTA and regular and white Portland cements at final concentration ranging from 1 to 1000 microg/mL for 1 h at 37 degrees C.
All compounds tested did not show genotoxic effects in all concentrations evaluated. No significant differences (P > .05) in cytotoxicity were observed for all compounds tested.
Taken together, our results suggest that MTA and Portland cements are not genotoxins and are not able to induce cellular death.
最近，三氧化矿物凝聚体（MTA）和波特兰水泥已被用作牙科根尖充填材料。然而，关于这些材料生物相容性的报道结果并不一致。本研究的目的是通过单细胞凝胶（彗星）试验和台盼蓝排斥试验，在体外检测MTA和波特兰水泥的遗传毒性和细胞毒性。
将中国仓鼠卵巢（CHO）细胞暴露于MTA、普通波特兰水泥和白色波特兰水泥中，终浓度范围为1至1000微克/毫升，在37℃下暴露1小时。
在所有评估浓度下，所有测试化合物均未显示遗传毒性作用。所有测试化合物的细胞毒性均未观察到显著差异（P>.05）。
综上所述，我们的结果表明，MTA和波特兰水泥不是遗传毒素，也不能诱导细胞死亡。
