Klinkert Ellen R, te Velde Egbert R, Weima Sjerp, van Zandvoort Peter M, Hanssen Rob G J M, Nilsson Philomeen R, de Jong Frank H, Looman Caspar W N, Broekmans Frank J M
Department of Reproductive Medicine, Division of Perinatology and Gynecology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
Reprod Biomed Online. 2006 Nov;13(5):687-95. doi: 10.1016/s1472-6483(10)60660-8.
Two very common single nucleotide polymorphisms at positions 307 and 680 in exon 10 of the FSH receptor gene have been associated with ovarian response in IVF. This observational study evaluated the role of the FSH receptor genotype in the prediction of poor response and clinical pregnancy in IVF in comparison with other markers, such as age, basal FSH, anti-Müllerian hormone and antral follicle count. In addition, the in-vitro cAMP response towards recombinant FSH in cultured granulosa cells of patients with different FSH receptor genotypes was determined. A total of 105 IVF patients undergoing ovarian stimulation in a long suppression protocol were included in the study. The ovarian response was comparable between patients with different FSH receptor genotypes. Patients with polymorphism Ser/Ser had implantation and pregnancy rates that were three times higher compared with patients with polymorphism Asn/Asn. FSH receptor genotype was not associated with a poor response in IVF, but showed a positive association with pregnancy, independent of age. There was no difference in cAMP production in cultured granulosa cells of patients with different FSH receptor genotypes (n=62). It is concluded that FSH receptor genotype is associated with pregnancy in IVF, but not with ovarian response.
促卵泡激素（FSH）受体基因第10外显子307和680位的两种非常常见的单核苷酸多态性与体外受精（IVF）中的卵巢反应有关。这项观察性研究评估了FSH受体基因型在预测IVF中反应不良和临床妊娠方面的作用，并与其他标志物（如年龄、基础FSH、抗苗勒管激素和窦卵泡计数）进行了比较。此外，还测定了不同FSH受体基因型患者培养的颗粒细胞对重组FSH的体外环磷酸腺苷（cAMP）反应。该研究共纳入了105例采用长方案抑制进行卵巢刺激的IVF患者。不同FSH受体基因型患者的卵巢反应相当。携带Ser/Ser多态性的患者与携带Asn/Asn多态性的患者相比，着床率和妊娠率高出三倍。FSH受体基因型与IVF中的反应不良无关，但与妊娠呈正相关，且与年龄无关。不同FSH受体基因型患者（n = 62）培养的颗粒细胞中cAMP的产生没有差异。研究得出结论，FSH受体基因型与IVF中的妊娠有关，但与卵巢反应无关。
