Departamento de Bioquímica, Biología Molecular y Celular, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain.
Unidad de Reproducción Asistida, Hospital Universitario Príncipe de Asturias, Universidad Complutense de Madrid, Alcalá de Henares, Madrid, Spain.
J Assist Reprod Genet. 2021 Oct;38(10):2769-2779. doi: 10.1007/s10815-021-02276-0. Epub 2021 Aug 3.
To determine the influence of different genotypes of Ala307Thr and Asn680Ser FSHr polymorphisms on controlled ovarian stimulation (COS) outcome and pregnancy.
This study collected blood and physiological and clinical parameters of 517 Caucasian patients (Statistical power ≥ 80%) that underwent COS treatment. Genotypes of Ala307Thr and Asn680Ser polymorphisms were determined using PCR amplification followed by Bsu36I and BsrI digestion, respectively.
Ala307Ala and Ser680Ser genotypes associated to worse parameters of COS outcome (preovulatory follicles P = 0.05, in both), justifying their lower pregnancy rate than Non-Ala307Ala, P = 0.01 and Non-Ser680Ser, P = 0.004, respectively or together, (P = 0.003). Within the Non-Ala307Ala group, Thr307Thr genotype showed higher number of fertilized oocytes (P = 0.04) and embryos (P = 0.01) than Non-Thr307Thr, but no influence on pregnancy rate. Ala307Ala and Ser680Ser patients doubled probability of non-pregnancy than Non-Ala307Ala (odds ratio = 2.0) and Non-Ser680Ser (odds ratio = 2.11), respectively. Ala307Ala and Ser680Ser genotypes tend to appear together (P < 0.0001), which increases the probability of non-pregnancy.
Ala307Ala and Ser680Ser genotypes of 307 and 680 FSHr polymorphisms associate to worse COS outcome than its respective Non-Ala307Ala and Non-Ser680Ser. Within the Non-Ala307Ala genotypes, Thr307Thr, although shows higher Fertilized Oocytes and Embryos, do not influence on pregnancy rate. Ala307Ala and Ser680Ser genotypes double the probability of Non-Pregnancy than their respective Non-Ala307Ala and Non-Ser680Ser genotypes. Furthermore, the strong tendency of these genotypes to appear together worsens the probability of pregnancy in these patients.
确定不同 Ala307Thr 和 Asn680Ser FSHr 多态性基因型对控制性卵巢刺激（COS）结局和妊娠的影响。
本研究收集了 517 名接受 COS 治疗的高加索患者的血液和生理及临床参数（统计效力≥80%）。使用聚合酶链反应（PCR）扩增后，分别用 Bsu36I 和 BsrI 消化确定 Ala307Thr 和 Asn680Ser 多态性的基因型。
Ala307Ala 和 Ser680Ser 基因型与 COS 结局的参数较差相关（P = 0.05，均），这解释了它们的妊娠率低于非-Ala307Ala（P = 0.01）和非-Ser680Ser（P = 0.004），或两者一起（P = 0.003）。在非-Ala307Ala 组中，Thr307Thr 基因型的受精卵（P = 0.04）和胚胎（P = 0.01）数量高于非-Thr307Thr，但对妊娠率没有影响。Ala307Ala 和 Ser680Ser 患者的非妊娠概率是非-Ala307Ala（比值比=2.0）和非-Ser680Ser（比值比=2.11）的两倍。Ala307Ala 和 Ser680Ser 基因型倾向于同时出现（P < 0.0001），这增加了非妊娠的可能性。
307 和 680 FSHr 多态性的 Ala307Ala 和 Ser680Ser 基因型与较差的 COS 结局相关，而非其各自的非-Ala307Ala 和非-Ser680Ser。在非-Ala307Ala 基因型中，虽然 Thr307Thr 显示出更高的受精卵和胚胎数量，但不影响妊娠率。Ala307Ala 和 Ser680Ser 基因型的非妊娠概率是其各自的非-Ala307Ala 和非-Ser680Ser 基因型的两倍。此外，这些基因型同时出现的强烈趋势恶化了这些患者的妊娠可能性。
