Cong Y Z, Rabin E, Wortis H H
Department of Pathology, Tufts University School of Medicine, Boston, MA 02111.
Int Immunol. 1991 May;3(5):467-76. doi: 10.1093/intimm/3.5.467.
Anti-Ig stimulated murine B cells express high levels of surface CD5 (ly-1) and increased CD44 while maintaining surface IgD, CD23 and J11d. Sorting of CD5- and CD5+ cells demonstrates that anti-Ig induces CD5 expression rather than the selective expansion of CD5+ cells. Anti Ig plus interleukin-6 (IL-6) induces the CD23, IgD, low ly-5 (B220) (CD45low), J11dhigh phenotype of typical CD5+ peritoneal B cells. In contrast, lipopolysaccharide (LPS)-stimulated B cells have high levels of CD44 but decreased surface IgD, CD23 and J11d and no CD5. Thus LPS and anti-Ig generate activated cells with differing phenotypes. Induced CD5+ cells have increased viability, even in the absence of added exogenous factors, while the viability of CD5- B cells is dependent on factors such as IL-4. We conclude that conventional CD5- B cells can be activated by either of two pathways: one generating CD5+ B cells; the other yielding conventional activated cells. We hypothesize that the first path requires slg cross-linking and corresponds to T-independent (type 2) stimulation, while cognate interaction with helper T cells in the absence of slg cross-linking induces B cells to enter the second path.
抗Ig刺激的鼠B细胞表达高水平的表面CD5（ly-1）且CD44增加，同时维持表面IgD、CD23和J11d。对CD5 - 和CD5 + 细胞进行分选表明，抗Ig诱导CD5表达而非CD5 + 细胞的选择性扩增。抗Ig加白细胞介素-6（IL-6）诱导典型CD5 + 腹膜B细胞的CD23、IgD、低水平ly-5（B220）（CD45low）、J11dhigh表型。相比之下，脂多糖（LPS）刺激的B细胞具有高水平的CD44，但表面IgD、CD23和J11d减少且无CD5。因此，LPS和抗Ig产生具有不同表型的活化细胞。诱导的CD5 + 细胞即使在没有添加外源性因子的情况下也具有增加的活力，而CD5 - B细胞的活力则依赖于诸如IL-4等因子。我们得出结论，传统的CD5 - B细胞可通过两种途径之一被激活：一种产生CD5 + B细胞；另一种产生传统的活化细胞。我们假设第一条途径需要表面免疫球蛋白（slg）交联，对应于非T细胞依赖性（2型）刺激，而在没有slg交联的情况下与辅助性T细胞的同源相互作用诱导B细胞进入第二条途径。
