Moyret C F, Bernard D J, Maurizis J C, Chassagne J, de Latour M, Betail G, Plagne R, Chollet P
Laboratoire d'Immunologie et de Cancérologie, Centre Jean Perrin, Clermont-Ferrand, France.
In Vivo. 1990 Mar-Apr;4(2):101-8.
In this study, the presence of reverse transcriptase in breast tumours was examined with immunoperoxidase staining using antibodies raised in rabbit against reverse transcriptase of Moloney murine leukemia virus and against reverse transcriptase of avian myeloblastosis virus. The specificity of such antibodies was investigated with ELISA and Western blotting techniques. Five cases of infiltrating ductal carcinomas were found positive with the immune serum anti-reverse transcriptase of Moloney murine leukemia virus on 28 studied infiltrating ductal carcinomas, 2 infiltrating lobular carcinomas and 2 fibroadenomas.
在本研究中，使用针对莫洛尼鼠白血病病毒逆转录酶和禽成髓细胞瘤病毒逆转录酶的兔源抗体，通过免疫过氧化物酶染色检测乳腺肿瘤中逆转录酶的存在。采用酶联免疫吸附测定法（ELISA）和蛋白质印迹法（Western blotting）研究此类抗体的特异性。在所研究的28例浸润性导管癌、2例浸润性小叶癌和2例纤维腺瘤中，发现5例浸润性导管癌对莫洛尼鼠白血病病毒逆转录酶免疫血清呈阳性。
