Graevskaia N A, Sito A F
Mol Biol (Mosk). 1976 May-Jun;10(2):652-6.
After immunization of rabbits the antiserum was prepared against purified reverse transcriptase (revertase) from avian myeloblastosis virus (AMV). The antiserum demonstrated enzymeneutralizing antibody activity that was associated with ummunoglobulin G fraction but not with IgM. The high antigenicity of AMV revertase for rabbits was shown. The active antiserum was obtained after 4 immunizations of rabbit with approximately 20 microgram of the enzyme. Non-specific revertase inhibitors were found in normal rabbit serum, which were absent in IgG fraction from this serum. The revertase activity of Rauscher leukemia virus (RLV) and Visna virus was not neutralized by antisera against AMV polymerase. This work was supported by the project "Revertase".
用禽成髓细胞瘤病毒（AMV）纯化的逆转录酶（反转酶）免疫兔子后制备抗血清。该抗血清表现出与免疫球蛋白G组分相关但与IgM无关的酶中和抗体活性。显示了AMV反转酶对兔子具有高抗原性。用约20微克该酶对兔子进行4次免疫后获得了活性抗血清。在正常兔血清中发现了非特异性反转酶抑制剂，而该血清的IgG组分中不存在这种抑制剂。抗AMV聚合酶的抗血清不能中和劳斯氏肉瘤病毒（RLV）和维斯纳病毒的反转酶活性。本研究得到“反转酶”项目的支持。
