Harmon S M, Kautter D A, Lancette G
Food and Drug Administration, Division of Microbiology, Washington, DC 20204.
J Assoc Off Anal Chem. 1991 Jul-Aug;74(4):649-51.
The use of the lipid globule stain to aid in differentiating the Bacillus cereus group (i.e., B. cereus, B. cereus var. mycoides, and B. thuringiensis) from other Bacillus species was investigated. Smears from colonies grown on suitable agar were made on precleaned slides, stained, and examined microscopically for characteristic deep blue lipid globules. The study included a total of 649 cultures of Bacillus species plus 143 incompletely characterized Bacillus isolates from food. Only B. cereus, B. cereus var. mycoides, B. thuringiensis, B. megaterium, and B. sphaericus were consistently positive for lipid globules, although at times, a few cells of B. aneurinolyticus and B. thiaminolyticus were also positive. The lipid globule stain procedure is of value in differentiating Bacillus species, especially when performed by an experienced analyst and used in conjunction with tests for cell and spore morphology.
研究了使用脂质球染色法来辅助区分蜡样芽孢杆菌群（即蜡样芽孢杆菌、蕈状芽孢杆菌变种和苏云金芽孢杆菌）与其他芽孢杆菌属物种。在预先清洁过的载玻片上制备来自在合适琼脂上生长的菌落的涂片，进行染色，并在显微镜下检查特征性的深蓝色脂质球。该研究总共包括649株芽孢杆菌属培养物以及143株来自食品的未完全鉴定的芽孢杆菌分离株。只有蜡样芽孢杆菌、蕈状芽孢杆菌变种、苏云金芽孢杆菌、巨大芽孢杆菌和球形芽孢杆菌始终呈现脂质球阳性，尽管有时，解脲芽孢杆菌和解硫胺素芽孢杆菌的少数细胞也呈阳性。脂质球染色程序在区分芽孢杆菌属物种方面具有价值，特别是由经验丰富的分析人员进行操作并与细胞和孢子形态测试结合使用时。
