Zhou Guoping, Liu Haizhou, He Jing, Yuan Yongming, Yuan Zhiming
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Int J Food Microbiol. 2008 Jan 31;121(2):195-200. doi: 10.1016/j.ijfoodmicro.2007.11.028. Epub 2007 Nov 17.
In 2006, a total of 54 samples of pasteurized full fat milk packaged in cartons were collected in spring and in autumn from chain supermarkets in Wuhan, China. The samples were examined and enumerated by MPN methods strictly according to guidelines laid out in US FDA/CFSAN BAM Chapter 14. Among 102 isolated B. cereus-like bacteria, 92 isolates were identified to be B. cereus, 9 B. thuringiensis and 1 B. mycoides. It was found that the occurrences of B. cereus were 71.4% and 33.3% in spring and in autumn samples respectively and the average count among the positive samples was 11.7 MPN/ml. The PCR detection results revealed that the enterotoxin genes hblA, hblC, hblD, nheA, nheB and nheC occurred in B. cereus isolates with frequencies of 37.0%, 66.3%, 71.7%, 71.7%, 62.0% and 71.7% respectively. Nine B. thuringiensis isolates were also identified from six pasteurized milk samples, and most of them harbored six enterotoxic genes and the insecticidal toxin cry1A gene. The single B. mycoides isolate harbored nheA and nheC genes. The data provides information for further evaluating the effect of B. cereus-like bacteria on food safety of Chinese milk products.
2006年，于春秋两季从中国武汉的连锁超市采集了54份纸盒包装的巴氏杀菌全脂牛奶样本。严格按照美国食品药品监督管理局/食品安全与应用营养中心《细菌分析手册》第14章规定的指南，采用MPN方法对样本进行检测和计数。在102株分离出的蜡样芽孢杆菌属样细菌中，92株鉴定为蜡样芽孢杆菌，9株为苏云金芽孢杆菌，1株为蕈状芽孢杆菌。结果发现，春季和秋季样本中蜡样芽孢杆菌的检出率分别为71.4%和33.3%，阳性样本中的平均菌数为11.7 MPN/ml。PCR检测结果显示，蜡样芽孢杆菌分离株中肠毒素基因hblA、hblC、hblD、nheA、nheB和nheC的出现频率分别为37.0%、66.3%、71.7%、71.7%、62.0%和71.7%。还从6份巴氏杀菌牛奶样本中鉴定出9株苏云金芽孢杆菌，其中大多数携带6种肠毒素基因和杀虫毒素cry1A基因。唯一的一株蕈状芽孢杆菌分离株携带nheA和nheC基因。这些数据为进一步评估蜡样芽孢杆菌属样细菌对中国奶制品食品安全的影响提供了信息。
