Stofer W D, Horn J P
Department of Physiology, University of Pittsburgh, School of Medicine, PA.
J Auton Nerv Syst. 1991 May;33(3):223-9. doi: 10.1016/0165-1838(91)90022-u.
Retrograde tracing in fixed tissue with the fluorescent carbocyanine dye, DiI, was used to identify neurons that project to paravertebral sympathetic ganglia 9 and 10 in bullfrog tadpoles. Applying DiI to ganglion 9 at stage II labelled spinal preganglionic neurons and sensory neurons in dorsal root ganglia. When examined in a stage V tadpole, the segmental boundaries of the preganglionic cell column which supply the lumbar chain ganglia were identical to that in the adult. Using immunocytochemistry, luteinizing hormone releasing hormone-like immunoreactivity, calcitonin gene-related peptide-like immunoreactivity, and substance P-like immunoreactivity were localized at stage III in axons within sympathetic ganglia 9 and 10. During subsequent stages, the density of fibers containing these peptides increased and it became easier to identify synaptic boutons in contact with postganglionic neurons. These observations demonstrate that projections to the lumbar sympathetic ganglia are already formed by the earliest tadpole stages, they are consistent with the previous physiological observation of nicotinic synapses in stage III ganglia, and they suggest that neuropeptide function may also begin during early stages.
利用荧光碳菁染料DiI对固定组织进行逆行追踪，以鉴定牛蛙蝌蚪中投射至第9和第10椎旁交感神经节的神经元。在II期将DiI应用于第9神经节，标记了脊髓节前神经元和背根神经节中的感觉神经元。在V期蝌蚪中检查时，供应腰链神经节的节前细胞柱的节段边界与成体中的相同。运用免疫细胞化学方法，在III期时，促黄体生成素释放激素样免疫反应性、降钙素基因相关肽样免疫反应性和P物质样免疫反应性定位于第9和第10交感神经节内的轴突中。在随后的阶段中，含有这些肽的纤维密度增加，并且更容易识别与节后神经元接触的突触小体。这些观察结果表明，到腰交感神经节的投射在蝌蚪最早阶段就已形成，它们与之前在III期神经节中对烟碱样突触的生理学观察结果一致，并且表明神经肽功能也可能在早期阶段就开始了。
