Horn J P, Stofer W D
Department of Physiology, University of Pittsburgh, School of Medicine, PA 15261.
J Auton Nerv Syst. 1988 Jun;23(1):17-24. doi: 10.1016/0165-1838(88)90162-2.
The specificity of synaptic contacts between pre- and postganglionic cells in the sympathetic C system has been examined by immunocytochemical localization of two neuropeptides. Sections of bullfrog paravertebral sympathetic ganglia were stained with antibodies to luteinizing hormone releasing hormone (LHRH) and neuropeptide Y (NPY). Preganglionic synaptic boutons containing LHRH immunoreactivity were found to make contact with a subpopulation of postganglionic cell bodies and with some clusters of small intensely fluorescent (SIF) cells. In ganglia 9 and 10, 95.8% of the neurons contacted by LHRH-containing boutons were also positive for NPY-like immunoreactivity and conversely, 99.3% of the neurons that contained NPY-like immunoreactivity were contacted by LHRH-containing boutons. Qualitatively similar results were found in most other paravertebral ganglia. These observations support the conclusions that preganglionic C axons selectively innervate C-type ganglion cells and that virtually all C-type ganglion cells and some SIF cells receive a direct LHRH input. Moreover, they suggest that a pattern of specific connections between two sets of peptidergic neurons is expressed throughout most of the paravertebral sympathetic chain of the bullfrog.
通过两种神经肽的免疫细胞化学定位，研究了交感C系统中节前和节后细胞之间突触联系的特异性。用抗促黄体生成素释放激素（LHRH）和神经肽Y（NPY）的抗体对牛蛙椎旁交感神经节切片进行染色。发现含有LHRH免疫反应性的节前突触终扣与节后细胞体的一个亚群以及一些小而强荧光（SIF）细胞簇接触。在第9和第10神经节中，被含LHRH终扣接触的神经元中有95.8%也对NPY样免疫反应呈阳性，反之，含有NPY样免疫反应的神经元中有99.3%被含LHRH终扣接触。在大多数其他椎旁神经节中也发现了定性相似的结果。这些观察结果支持以下结论：节前C轴突选择性地支配C型神经节细胞，并且几乎所有C型神经节细胞和一些SIF细胞都接受直接的LHRH输入。此外，它们表明在牛蛙的大部分椎旁交感神经链中，两组肽能神经元之间存在特定的连接模式。
