Yamamoto K, Senba E, Matsunaga T, Tohyama M
Department of Otolaryngology, Osaka University, Medical School, Japan.
Brain Res. 1989 May 15;487(1):158-64. doi: 10.1016/0006-8993(89)90952-9.
The origin of calcitonin gene-related peptide (CGRP)-containing fibers observed in the superior cervical ganglia (SCG) of the rat was investigated by a combined technique of retrograde axonal tracing and indirect immunofluorescence. Following the injection of Fast blue (FB) into the SCG, labeled neurons were observed in the C8-T5 spinal cord segments, with the highest density in T1-T3 (5-8 neurons per section). More than 90% of them were located in the ipsilateral intermediolateral cell column (IML) and the rest were found in the central autonomic area (CA) and intercalated region (IC) between the IML and CA. CGRP-like immunoreactive (IR) neurons were detected in these areas in animals pretreated with colchicine. About one-fourth of FB-labeled cells were CGRP-IR, which corresponded to three-fourths of the CGRP-IR neurons in the above-mentioned autonomic areas of these spinal cord segments. Most of these double-labeled cells were found in the IML (95%). A few FB-labeled cells were also observed in dorsal root ganglia (C8-T5) and 30% of them were CGRP-IR. These findings suggested that the CGRP-IR fibers in the rats SCG are supplied from both sympathetic preganglionic neurons in the spinal cord and sensory ganglion cells, although the latter projection is quite rare.
采用逆行轴突追踪和间接免疫荧光相结合的技术，研究了大鼠颈上神经节（SCG）中降钙素基因相关肽（CGRP）阳性纤维的起源。将快蓝（FB）注入SCG后，在C8 - T5脊髓节段观察到标记神经元，其中T1 - T3节段密度最高（每节5 - 8个神经元）。超过90%的标记神经元位于同侧中间外侧细胞柱（IML），其余位于中央自主区（CA）以及IML与CA之间的中间区（IC）。在秋水仙碱预处理的动物中，这些区域检测到CGRP样免疫反应性（IR）神经元。约四分之一的FB标记细胞为CGRP - IR，这相当于上述脊髓节段自主区中CGRP - IR神经元的四分之三。这些双重标记细胞大多位于IML（95%）。在背根神经节（C8 - T5）中也观察到少数FB标记细胞，其中30%为CGRP - IR。这些结果表明，大鼠SCG中的CGRP - IR纤维来自脊髓中的交感神经节前神经元和感觉神经节细胞，尽管后者的投射非常少见。
