Perrella O, Sbreglia C, Arenga G, Perrella A, Ferrara A, D'Antonio A, Di Costanzo G, Atripaldi L, Alone C, Sciano D, Cuomo O
Department of Laparascopic, Hepatic Surgery and Liver Transplant Unit, AORN, A. Cardarelli Hospital, Naples, Italy.
Transplant Proc. 2006 Dec;38(10):3594-6. doi: 10.1016/j.transproceed.2006.10.102.
The aim of the study was to assess various T-cell subsets and cytokine secretion patterns both in liver tissue and in the peripheral blood of 24 liver transplant patients to assess possible specific immunological involvement in early acute rejection episodes after liver transplantation. Particularly, we studied CD4+ CD7+, CD8+ CD38+, and CD4+ CD25+ T cells by flow cytometry, as well as contemporaneously, interleukin (IL)-2 and IL-10 secretion by ELISpot to determine possible Th1-like immune responses and the immunomodulation expressed by Treg cells in acute liver rejection, respectively. As a control group we included patients transplanted without acute rejection. Early acute rejection within the first 4 weeks was proven histologically in 42% of patients. It was associated with a greater expression of CD4+ CD7+ and CD8+ CD38+ T cells in the liver than in the blood (P < .001). A contemporaneous reduced expansion of liver Treg cells was evident in patients with acute rejection (P < .001). Our data suggested that a preferential Th1-like immune mechanism operated in local fashion as characterized by a decreased presence in the liver and blood of Treg cells.
该研究旨在评估24例肝移植患者肝脏组织和外周血中的各种T细胞亚群及细胞因子分泌模式，以评估肝移植术后早期急性排斥反应中可能存在的特异性免疫参与情况。具体而言，我们通过流式细胞术研究了CD4+ CD7+、CD8+ CD38+和CD4+ CD25+ T细胞，同时通过酶联免疫斑点法检测白细胞介素（IL）-2和IL-10的分泌，分别以确定急性肝排斥反应中可能存在的Th1样免疫反应以及调节性T细胞（Treg）所表达的免疫调节作用。作为对照组，我们纳入了未发生急性排斥反应的移植患者。42%的患者在术后4周内被组织学证实发生了早期急性排斥反应。与血液相比，肝脏中CD4+ CD7+和CD8+ CD38+ T细胞的表达更高（P <.001）。急性排斥反应患者的肝脏Treg细胞同时出现明显的增殖减少（P <.001）。我们的数据表明，一种以肝脏和血液中Treg细胞数量减少为特征的、以局部方式起作用的Th1样免疫机制发挥了作用。
