Gregson Aric L, Hoji Aki, Saggar Rajan, Ross David J, Kubak Bernard M, Jamieson Beth D, Weigt S Samuel, Lynch Joseph P, Ardehali Abbas, Belperio John A, Yang Otto O
Division of Infectious Diseases, Department of Medicine, University of California, Los Angeles, CA 90095, USA.
Transplantation. 2008 Apr 15;85(7):1056-9. doi: 10.1097/TP.0b013e318169bd85.
Bronchoalveolar lavage fluid (BALF) offers a potential means to diagnose acute rejection and could provide insight into the immune mechanisms responsible for lung allograft rejection. Transbronchial biopsies from 29 bronchoscopic procedures were assessed for rejection. Concurrent BALF lymphocyte subsets were examined by flow cytometry, including CD4 and CD8 T cells and their activation status by CD38 expression, natural killer (NK), NK-like T (NT), B, regulatory T, and invariant receptor NK-T cells. Percentages of CD4 were reduced, and CD8 and activation of CD4 T cells correlated with rejection. There were trends for increased NT, reduced NK, and increased B cell percentages with rejection, suggesting potential roles of these cells. Among regulatory cells, the percentages of regulatory T cells decreased and CD4/CD8 invariant NK-T cells increased during rejection, suggesting a proinflammatory profile. A unique BALF lymphocyte profile was associated with rejection and may provide insight into the pathogenesis of allograft rejection.
支气管肺泡灌洗术（BALF）为诊断急性排斥反应提供了一种潜在方法，并且有助于深入了解导致肺移植排斥反应的免疫机制。对29例支气管镜检查获取的经支气管活检组织进行排斥反应评估。同时，通过流式细胞术检测BALF淋巴细胞亚群，包括CD4和CD8 T细胞及其通过CD38表达反映的激活状态、自然杀伤（NK）细胞、自然杀伤样T（NT）细胞、B细胞、调节性T细胞和不变受体NK-T细胞。CD4百分比降低，CD8以及CD4 T细胞的激活与排斥反应相关。NT细胞百分比增加、NK细胞百分比降低以及B细胞百分比增加与排斥反应存在一定趋势，提示这些细胞可能发挥作用。在调节性细胞中，排斥反应期间调节性T细胞百分比降低，CD4/CD8不变NK-T细胞百分比增加，提示存在促炎状态。独特的BALF淋巴细胞谱与排斥反应相关，可能有助于深入了解移植排斥反应的发病机制。
