Carraro Emerson, Neto Daniel Ferreira, Benfica Daniela, SittaPerosa Ana Helena, Granato Celso Francisco Hernandes, Bellei Nancy Cristina Junqueira
Clinical Virology Laboratory, Infectious Diseases Unit, Medicine Department, São Paulo Federal University, Rua Pedro de Toledo, CEP: 04039-032, São Paulo, SP, Brazil.
Diagn Microbiol Infect Dis. 2007 Jan;57(1):53-7. doi: 10.1016/j.diagmicrobio.2006.11.004.
A duplex reverse transcription polymerase chain reaction (RT-PCR) and direct immunofluorescence assays (DFAs) were evaluated for detection of influenza types A and B in comparison with virus isolation in Madin-Darbin canine kidney cells. Four hundred four nasal wash were collected from individuals presenting with acute respiratory symptoms during 2001 to 2003 influenza seasons. According to the reference method, 78 (19.3%) samples were infected by influenza virus: 46 were type A and 32 type B. The overall concordance between the 3 assays was 96%, with 317 negative and 71 positive samples in all tests. RT-PCR reached 92.3% sensitivity and 98.5% specificity, and for DFA, the corresponding values were 93.6% and 97.2%, respectively. DFA and RT-PCR could be applied in different routine settings, resulting as an advantage compared with virus isolation: DFA provides rapid results for clinical purposes, but RT-PCR allows running more samples, an important concern in early pandemic circumstances.
对双重逆转录聚合酶链反应（RT-PCR）和直接免疫荧光测定法（DFA）进行了评估，以检测甲型和乙型流感病毒，并与在麦迪逊-达比犬肾细胞中进行病毒分离的方法进行比较。在2001年至2003年流感季节期间，从出现急性呼吸道症状的个体中收集了404份鼻洗液。根据参考方法，78份（19.3%）样本感染了流感病毒：46份为甲型，32份为乙型。这三种检测方法的总体一致性为96%，所有检测中317份样本为阴性，71份样本为阳性。RT-PCR的灵敏度达到92.3%，特异性达到98.5%，而DFA的相应值分别为93.6%和97.2%。DFA和RT-PCR可应用于不同的常规检测，与病毒分离相比具有优势：DFA可为临床目的提供快速结果，但RT-PCR可检测更多样本，这在早期大流行情况下是一个重要问题。
