Zheng Weifa, Gao Xiaowen, Gu Qi, Chen Caifa, Wei Zhiwen, Shi Feng
Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou, China.
Int Immunopharmacol. 2007 Feb;7(2):128-34. doi: 10.1016/j.intimp.2006.07.011. Epub 2006 Aug 2.
Daphne genkwa root has been traditionally used as an effective remedy to treat various tumors. However, the active constituents for its antitumor potency have not been well documented. During the screening for antitumor constituents, it was found that daphnodorins were responsible for the inhibition of tumor growth and metastasis. In this study, six daphnodorins including daphnodorins B (1), G (2), H (3), H-3''-methylether (4), H-3-methylether (5) and G-3''-methylether (6) were investigated for the protection against LLC-induced reduction of lymphoid organs and peripheral lymphocytes, and for the activities against tumor growth and metastasis. The six daphnodorins showed selective cytotoxicity to a number of tumor cell lines. Treatment of LLC-bearing mice with daphnodorin B and/or daphnodorin complex evidently protected peripheral lymphocytes from tumor-induced reduction, increased lymphocyte proliferation potential and inhibited tumor progression and metastasis at doses of 40 and 80 mg/kg. These results indicated that daphnodorin B or daphnodorin complex inhibited tumor growth and metastasis by protecting host immunocyte viability and proliferation potential, and selectively inhibiting tumor cell proliferation.
芫花根传统上被用作治疗各种肿瘤的有效药物。然而，其抗肿瘤效力的活性成分尚未得到充分记载。在筛选抗肿瘤成分的过程中，发现瑞香毒素是抑制肿瘤生长和转移的原因。在本研究中，研究了六种瑞香毒素，包括瑞香毒素B（1）、G（2）、H（3）、H-3''-甲醚（4）、H-3-甲醚（5）和G-3''-甲醚（6）对LLC诱导的淋巴器官和外周淋巴细胞减少的保护作用，以及对肿瘤生长和转移的活性。这六种瑞香毒素对多种肿瘤细胞系表现出选择性细胞毒性。用瑞香毒素B和/或瑞香毒素复合物处理荷LLC小鼠，在40和80mg/kg剂量下，明显保护外周淋巴细胞免受肿瘤诱导的减少，增加淋巴细胞增殖潜能，并抑制肿瘤进展和转移。这些结果表明，瑞香毒素B或瑞香毒素复合物通过保护宿主免疫细胞活力和增殖潜能以及选择性抑制肿瘤细胞增殖来抑制肿瘤生长和转移。
